← Back to Zoology

Biotechnology And Its Applications

339 Questions Start Allen Test
All Exams Allen
Q26 Allen PRINCIPLES AND PROCESSES MCQ
The Ti plasmid, is often used for making transgenic plants. This plasmid is found in :-
A.
Yeast
B.
Azotobacter
C.
Rhizobium
D.
Agrobacterium
Q27 Allen PRINCIPLES AND PROCESSES MCQ
Which of the following is the example of direct gene transfer?
A.
Microinjection
B.
Electroporation
C.
Particle gun
D.
All the above
Q28 Allen PRINCIPLES AND PROCESSES MCQ
How many copies of DNA sample are produced in "PCR" technique after 6ā€“cycle?
A.
4
B.
32
C.
64
D.
16
Q29 Allen PRINCIPLES AND PROCESSES MCQ
Thermal cycle takes place in which technique
A.
Gel electrophoresis
B.
"PCR"-technique
C.
Centrifugation
D.
Southern blotting
Q30 Allen PRINCIPLES AND PROCESSES MCQ
BAC and YAC are :-
A.
Natural DNA obtained from bacteria and yeast
B.
Useful vectors for eukaryotic gene transfer
C.
Artificial DNA obtained from bacteria and yeast
D.
2 & 3 both
Q31 Allen PRINCIPLES AND PROCESSES MCQ
Restriction enzymes are :-
A.
Not always required in genetic engineering
B.
Essential tool in genetic engineering
C.
Nucleases that cleave DNA at specific sites
D.
2 and 3 both
Q32 Allen PRINCIPLES AND PROCESSES MCQ
Electroporation procedure involves :-
A.
Fast passage of food through sieve pores in phloem elements with the help of electric stimulation.
B.
Opening of stomatal pores during night by artificial light
C.
Making transient pores in the cell membrane to introduce gene constructs
D.
Purification of saline water with the help
of a membrane system.
Q33 Allen PRINCIPLES AND PROCESSES MCQ
Which of the following restriction endonuclease enzyme produce blunt end in DNA
A.
image.png
B.
image.png
C.
image.png
D.
All the above
Q34 Allen PRINCIPLES AND PROCESSES MCQ
A bacterium modifies its DNA by adding methyl groups to the DNA, It does so to :-
A.
Clone its DNA
B.
Be able to transcribe many genes simultaneously
C.
Turn its gene on
D.
Protect its DNA from its own restriction enzyme
Q35 Allen PRINCIPLES AND PROCESSES MCQ
The restriction enzyme Eco RI has the property of:-
A.
endonuclease activity
B.
exonuclease activity
C.
ligation activity
D.
correcting the topology of replicating DNA
Q36 Allen PRINCIPLES AND PROCESSES MCQ
DNA ligase is an enzyme that catalyses the:-
A.
splitting of DNA threads into small bits
B.
joining of the fragments of DNA
C.
denaturation of DNA
D.
synthesis of DNA
Q37 Allen PRINCIPLES AND PROCESSES MCQ
Agrobacterium tumefaciens contains a large plasmid, which induces tumour in the plants it is termed as :-
A.
Ti plasmid
B.
Ri plasmid
C.
Recombinant plasmid
D.
Shine Delgrano sequence
Q38 Allen PRINCIPLES AND PROCESSES MCQ
More advancement in genetic engineering is due to :-
A.
Restriction endonuclease
B.
Reverse transcriptase
C.
Protease
D.
Zymase
Q39 Allen PRINCIPLES AND PROCESSES MCQ
Which of the follwing is used as a best genetic vector in plants?
A.
Bacillus thuriengenesis
B.
Agrobacterium tumifaciens
C.
Pseudomonas putida
D.
All of these
Q40 Allen PRINCIPLES AND PROCESSES MCQ
Which of the following enzyme is used to join DNA fragments?
A.
Terminase
B.
Endonuclease
C.
Ligase
D.
DNA polymerase
Q41 Allen PRINCIPLES AND PROCESSES MCQ
A kind of Biotechnology involving manipulation of DNA is :-
A.
DNA replication
B.
Genetic engineering
C.
Denaturation
D.
Renaturation
Q42 Allen PRINCIPLES AND PROCESSES MCQ
What is true of plasmid ?
A.
Found in viruses
B.
Contains genes for vital activities
C.
Part of nuclear chromosome
D.
Widely used in gene transfer
Q43 Allen PRINCIPLES AND PROCESSES MCQ
A suitable vector for gene cloning in higher organism is :-
A.
Baculovirus
B.
Retrovirus
C.
Salmonella typhimurium
D.
Neurospora crassa
Q44 Allen PRINCIPLES AND PROCESSES MCQ
"PCR" proceeds in three distinct steps governed by temperature they are in order of:-
A.
Denaturation, Annealing, Synthesis
B.
Synthesis, Annealing, Denaturation
C.
Annealing, Synthesis, Denaturation
D.
Denaturation, Synthesis, Annealing
Q45 Allen PRINCIPLES AND PROCESSES MCQ
What is the source of the Ti (Tumor inducing) plasmid which is modified and used as a cloning vector to deliver the desirable genes into plant cells ?
A.
Agrobacterium tumifaciens
B.
Thermophilus aquaticus
C.
Pyrococcus furiosus
D.
Aedes aegypti
Q46 Allen PRINCIPLES AND PROCESSES MCQ
The thermostable enzymes, 'Taq' isolated from thermophilic bacteria is :-
A.
RNA polymerases
B.
DNA polymerases
C.
Restriction endonucleases
D.
DNA ligases
Q47 Allen PRINCIPLES AND PROCESSES MCQ
The term "molecular scissors" generally refers to:-
A.
DNA polymerases
B.
RNA polymerases
C.
Restriction endonucleases
D.
DNA ligases
Q48 Allen PRINCIPLES AND PROCESSES MCQ
In the "PCR" technology the DNA segment is replicated over a billion times. This repeated replications catalyzed by the enzyme :-
A.
RNA polymerase
B.
Taq polymerase
C.
DNA dependent RNA polymerase
D.
Primase
Q49 Allen PRINCIPLES AND PROCESSES MCQ
The restriction enzyme(s) used in recombinant DNA technology making staggered cuts in DNA leaving sticky ends is/are :-
A.
EcoR I
B.
Hind III
C.
BamH I
D.
All of the above
Q50 Allen PRINCIPLES AND PROCESSES MCQ
Restriction enzyme Eco RI cuts the DNA between bases G and A only when the sequence in DNA is:-
A.
GATATC
B.
GAATTC
C.
GATTCC
D.
GAACTT