Biotechnology And Its Applications

339 Questions Start Allen Test
1990 Q1 Allen PYQ MCQ
Which kind of therapy was given in 1990 to a four year old girl with adenosine deaminase (ADA) deficiency ?
A.
Immunotherapy
B.
Radiation therapy
C.
Gene therapy
D.
Chemotherapy
1984 Q2 Allen NCERT BASED QUESTIONS MCQ
The first clinical gene therapy was given to a 4-year old girl with ADA deficiency in :-
A.
1984
B.
1986
C.
1992
D.
1990
1968 Q3 Allen NCERT BASED QUESTIONS MCQ
First recombinant DNA was made by Stanley Cohen and Herbert Boyer in :-
A.
1968
B.
1970
C.
1972
D.
1974
1963 Q4 Allen NCERT BASED QUESTIONS MCQ
The enzymes responsible for restricting the growth of bacteriophage in E coli were isolated in 1963, these enzyme are :-
A.
DNA ligases
B.
Alkaline phosphatases
C.
DNA polymerases
D.
Restriction endonuclease
1963 Q5 Allen NCERT BASED QUESTIONS MCQ
Two enzymes responsible for restricting the growth of bacteriophage in E. coli were isolated in 1963, one of these cut DNA, while other :-
A.
Add propyl group to DNA
B.
Add ethyl group to DNA
C.
Add methyl group to DNA
D.
None of the above
Q6 Allen PRINCIPLES AND PROCESSES MCQ
Agrobacterium tumefaciens used in Genetic engineering is for :-
A.
DNA-mapping
B.
DNA-modification
C.
Gene transfer
D.
DNA finger printing
Q7 Allen PRINCIPLES AND PROCESSES MCQ
Who isolated the first restriction endonucleases?
A.
Temin & Baltimore
B.
Sanger
C.
Smith
D.
Paul berg
Q8 Allen PRINCIPLES AND PROCESSES MCQ
Genetic engineering is :-
A.
Study of extra nuclear gene
B.
Manipulation of genotype by artificial method
C.
Manipulation of RNA
D.
Manipulation of enzymes
Q9 Allen PRINCIPLES AND PROCESSES MCQ
Polymerase chain reaction technology ("PCR"-technique) is used for :-
A.
DNA identification
B.
DNA repair
C.
DNA amplification
D.
Cleave DNA
Q10 Allen PRINCIPLES AND PROCESSES MCQ
Which structure involved in genetic engineering?
A.
Plastid
B.
Plasmid
C.
Codon
D.
None
Q11 Allen PRINCIPLES AND PROCESSES MCQ
Which of the following is the example of chemical scissors?
A.
Eco - RI
B.
Hind - III
C.
Bam - I
D.
All the above
Q12 Allen PRINCIPLES AND PROCESSES MCQ
Restriction endonucleases are used in genetic engineering because:-
A.
They can degrade harmful proteins
B.
They can join DNA fragments
C.
They can cut DNA at variable site
D.
They can cut DNA at specific base sequences
Q13 Allen PRINCIPLES AND PROCESSES MCQ
When the genotype of an organism is improved by the addition of foreign gene, the process is called :-
A.
Tissue culture
B.
Genetic diversity
C.
Genetic engineering
D.
Plastic surgery
Q14 Allen PRINCIPLES AND PROCESSES MCQ
A genetically manipulated organism containing in its genome one or more inserted gene of another species is called :-
A.
Transposon
B.
Gene expression
C.
Transgenic organism
D.
Retroposons
Q15 Allen PRINCIPLES AND PROCESSES MCQ
Which vector is commonly used in the transfer of gene in a crop plant?
A.
Plasmids of B. Subtilis
B.
Bacteriophages
C.
Ti-plasmids of Agrobacterium
D.
E. coli Phages
Q16 Allen PRINCIPLES AND PROCESSES MCQ
The tumour inducing capacity of Agrobacterium tumefaciens is located in extrachromosomal plasmid and called:-
A.
Ti – plasmid
B.
Ri - plasmid
C.
Lambda phage
D.
Plasmid $p_{BR}$ 322
Q17 Allen PRINCIPLES AND PROCESSES MCQ
Genetic engineering aims at :-
A.
Destroying wild gene
B.
Preserving defective gene
C.
Curing human disease by introducing new gene
D.
All the above
Q18 Allen PRINCIPLES AND PROCESSES MCQ
A piece of nucleic acid using to find out a gene, by forming hybrid with it, is called as:-
A.
c – DNA
B.
DNA probe
C.
Sticky end
D.
Blunt end
Q19 Allen PRINCIPLES AND PROCESSES MCQ
Taq - polymerase which is used for amplification of DNA is related with:-
A.
Hybridoma technique
B.
""-technique
C.
Gene cloning
D.
r-DNA technology
Q20 Allen PRINCIPLES AND PROCESSES MCQ
What is true for plasmid?
A.
Plasmids are widely used in gene transfer
B.
These are found in virus
C.
Plasmid contains gene for vital activities
D.
These are main part of chromosome
Q21 Allen PRINCIPLES AND PROCESSES MCQ
Which of the following cuts the DNA from specific places?
A.
Restriction endonuclease
B.
Ligase
C.
Exonuclease
D.
Alkaline phosphatase
Q22 Allen PRINCIPLES AND PROCESSES MCQ
Manipulation of DNA in genetic engineering became possible due to the discovery of:-
A.
Restriction endonuclease
B.
DNA ligase
C.
Transcriptase
D.
Primase
Q23 Allen PRINCIPLES AND PROCESSES MCQ
Which one of the following has found extensive use in genetic engineering work in plants?
A.
Bacillus coagulens
B.
Agrobacterium tumefaciens.
C.
Clostridium septicum
D.
Xanthomonas citri
Q24 Allen PRINCIPLES AND PROCESSES MCQ
Restriction endonucleases: -
A.
Are enzymes which cleave DNA at specific sites
B.
Make DNA complementary to an existing DNA or RNA
C.
Cut or join DNA fragments
D.
Are required in vectorless direct gene transfer.
Q25 Allen PRINCIPLES AND PROCESSES MCQ
Restriction endonucleases :-
A.
Are synthesized by bacteria as part of their defense mechanism
B.
Are present in mammalian cells for degradation of DNA when the cell dies
C.
Are used in genetic engineering for ligating two DNA molecules
D.
Are used for invitro DNA synthesis