Question 1: Palette 1/339

Allen 1990 MCQ

Which kind of therapy was given in 1990 to a four year old girl with adenosine deaminase (ADA) deficiency ?

A

Immunotherapy

B

Radiation therapy

C

Gene therapy

D

Chemotherapy

Question 2: Palette 2/339

Allen 1984 MCQ

The first clinical gene therapy was given to a 4-year old girl with ADA deficiency in :-

A

1984

B

1986

C

1992

D

1990

Question 3: Palette 3/339

Allen 1968 MCQ

First recombinant DNA was made by Stanley Cohen and Herbert Boyer in :-

A

1968

B

1970

C

1972

D

1974

Question 4: Palette 4/339

Allen 1963 MCQ

The enzymes responsible for restricting the growth of bacteriophage in E coli were isolated in 1963, these enzyme are :-

A

DNA ligases

B

Alkaline phosphatases

C

DNA polymerases

D

Restriction endonuclease

Question 5: Palette 5/339

Allen 1963 MCQ

Two enzymes responsible for restricting the growth of bacteriophage in E. coli were isolated in 1963, one of these cut DNA, while other :-

A

Add propyl group to DNA

B

Add ethyl group to DNA

C

Add methyl group to DNA

D

None of the above

Question 6: Palette 6/339

Allen MCQ

Agrobacterium tumefaciens used in Genetic engineering is for :-

A

DNA-mapping

B

DNA-modification

C

Gene transfer

D

DNA finger printing

Question 7: Palette 7/339

Allen MCQ

Who isolated the first restriction endonucleases?

A

Temin & Baltimore

B

Sanger

C

Smith

D

Paul berg

Question 8: Palette 8/339

Allen MCQ

Genetic engineering is :-

A

Study of extra nuclear gene

B

Manipulation of genotype by artificial method

C

Manipulation of RNA

D

Manipulation of enzymes

Question 9: Palette 9/339

Allen MCQ

Polymerase chain reaction technology ("PCR"-technique) is used for :-

A

DNA identification

B

DNA repair

C

DNA amplification

D

Cleave DNA

Question 10: Palette 10/339

Allen MCQ

Which structure involved in genetic engineering?

A

Plastid

B

Plasmid

C

Codon

D

None

Question 11: Palette 11/339

Allen MCQ

Which of the following is the example of chemical scissors?

A

Eco - RI

B

Hind - III

C

Bam - I

D

All the above

Question 12: Palette 12/339

Allen MCQ

Restriction endonucleases are used in genetic engineering because:-

A

They can degrade harmful proteins

B

They can join DNA fragments

C

They can cut DNA at variable site

D

They can cut DNA at specific base sequences

Question 13: Palette 13/339

Allen MCQ

When the genotype of an organism is improved by the addition of foreign gene, the process is called :-

A

Tissue culture

B

Genetic diversity

C

Genetic engineering

D

Plastic surgery

Question 14: Palette 14/339

Allen MCQ

A genetically manipulated organism containing in its genome one or more inserted gene of another species is called :-

A

Transposon

B

Gene expression

C

Transgenic organism

D

Retroposons

Question 15: Palette 15/339

Allen MCQ

Which vector is commonly used in the transfer of gene in a crop plant?

A

Plasmids of B. Subtilis

B

Bacteriophages

C

Ti-plasmids of Agrobacterium

D

E. coli Phages

Question 16: Palette 16/339

Allen MCQ

The tumour inducing capacity of Agrobacterium tumefaciens is located in extrachromosomal plasmid and called:-

A

Ti – plasmid

B

Ri - plasmid

C

Lambda phage

D

Plasmid $p_{BR}$ 322

Question 17: Palette 17/339

Allen MCQ

Genetic engineering aims at :-

A

Destroying wild gene

B

Preserving defective gene

C

Curing human disease by introducing new gene

D

All the above

Question 18: Palette 18/339

Allen MCQ

A piece of nucleic acid using to find out a gene, by forming hybrid with it, is called as:-

A

c – DNA

B

DNA probe

C

Sticky end

D

Blunt end

Question 19: Palette 19/339

Allen MCQ

Taq - polymerase which is used for amplification of DNA is related with:-

A

Hybridoma technique

B

""-technique

C

Gene cloning

D

r-DNA technology

Question 20: Palette 20/339

Allen MCQ

What is true for plasmid?

A

Plasmids are widely used in gene transfer

B

These are found in virus

C

Plasmid contains gene for vital activities

D

These are main part of chromosome

Question 21: Palette 21/339

Allen MCQ

Which of the following cuts the DNA from specific places?

A

Restriction endonuclease

B

Ligase

C

Exonuclease

D

Alkaline phosphatase

Question 22: Palette 22/339

Allen MCQ

Manipulation of DNA in genetic engineering became possible due to the discovery of:-

A

Restriction endonuclease

B

DNA ligase

C

Transcriptase

D

Primase

Question 23: Palette 23/339

Allen MCQ

Which one of the following has found extensive use in genetic engineering work in plants?

A

Bacillus coagulens

B

Agrobacterium tumefaciens.

C

Clostridium septicum

D

Xanthomonas citri

Question 24: Palette 24/339

Allen MCQ

Restriction endonucleases: -

A

Are enzymes which cleave DNA at specific sites

B

Make DNA complementary to an existing DNA or RNA

C

Cut or join DNA fragments

D

Are required in vectorless direct gene transfer.

Question 25: Palette 25/339

Allen MCQ

Restriction endonucleases :-

A

Are synthesized by bacteria as part of their defense mechanism

B

Are present in mammalian cells for degradation of DNA when the cell dies

C

Are used in genetic engineering for ligating two DNA molecules

D

Are used for invitro DNA synthesis

Question 26: Palette 26/339

Allen MCQ

The Ti plasmid, is often used for making transgenic plants. This plasmid is found in :-

A

Yeast

B

Azotobacter

C

Rhizobium

D

Agrobacterium

Question 27: Palette 27/339

Allen MCQ

Which of the following is the example of direct gene transfer?

A

Microinjection

B

Electroporation

C

Particle gun

D

All the above

Question 28: Palette 28/339

Allen MCQ

How many copies of DNA sample are produced in "PCR" technique after 6–cycle?

A

4

B

32

C

64

D

16

Question 29: Palette 29/339

Allen MCQ

Thermal cycle takes place in which technique

A

Gel electrophoresis

B

"PCR"-technique

C

Centrifugation

D

Southern blotting

Question 30: Palette 30/339

Allen MCQ

BAC and YAC are :-

A

Natural DNA obtained from bacteria and yeast

B

Useful vectors for eukaryotic gene transfer

C

Artificial DNA obtained from bacteria and yeast

D

2 & 3 both

Question 31: Palette 31/339

Allen MCQ

Restriction enzymes are :-

A

Not always required in genetic engineering

B

Essential tool in genetic engineering

C

Nucleases that cleave DNA at specific sites

D

2 and 3 both

Question 32: Palette 32/339

Allen MCQ

Electroporation procedure involves :-

A

Fast passage of food through sieve pores in phloem elements with the help of electric stimulation.

B

Opening of stomatal pores during night by artificial light

C

Making transient pores in the cell membrane to introduce gene constructs

D

Purification of saline water with the help
of a membrane system.

Question 33: Palette 33/339

Allen MCQ

Which of the following restriction endonuclease enzyme produce blunt end in DNA

A

B

C

D

All the above

Question 34: Palette 34/339

Allen MCQ

A bacterium modifies its DNA by adding methyl groups to the DNA, It does so to :-

A

Clone its DNA

B

Be able to transcribe many genes simultaneously

C

Turn its gene on

D

Protect its DNA from its own restriction enzyme

Question 35: Palette 35/339

Allen MCQ

The restriction enzyme Eco RI has the property of:-

A

endonuclease activity

B

exonuclease activity

C

ligation activity

D

correcting the topology of replicating DNA

Question 36: Palette 36/339

Allen MCQ

DNA ligase is an enzyme that catalyses the:-

A

splitting of DNA threads into small bits

B

joining of the fragments of DNA

C

denaturation of DNA

D

synthesis of DNA

Question 37: Palette 37/339

Allen MCQ

Agrobacterium tumefaciens contains a large plasmid, which induces tumour in the plants it is termed as :-

A

Ti plasmid

B

Ri plasmid

C

Recombinant plasmid

D

Shine Delgrano sequence

Question 38: Palette 38/339

Allen MCQ

More advancement in genetic engineering is due to :-

A

Restriction endonuclease

B

Reverse transcriptase

C

Protease

D

Zymase

Question 39: Palette 39/339

Allen MCQ

Which of the follwing is used as a best genetic vector in plants?

A

Bacillus thuriengenesis

B

Agrobacterium tumifaciens

C

Pseudomonas putida

D

All of these

Question 40: Palette 40/339

Allen MCQ

Which of the following enzyme is used to join DNA fragments?

A

Terminase

B

Endonuclease

C

Ligase

D

DNA polymerase

Question 41: Palette 41/339

Allen MCQ

A kind of Biotechnology involving manipulation of DNA is :-

A

DNA replication

B

Genetic engineering

C

Denaturation

D

Renaturation

Question 42: Palette 42/339

Allen MCQ

What is true of plasmid ?

A

Found in viruses

B

Contains genes for vital activities

C

Part of nuclear chromosome

D

Widely used in gene transfer

Question 43: Palette 43/339

Allen MCQ

A suitable vector for gene cloning in higher organism is :-

A

Baculovirus

B

Retrovirus

C

Salmonella typhimurium

D

Neurospora crassa

Question 44: Palette 44/339

Allen MCQ

"PCR" proceeds in three distinct steps governed by temperature they are in order of:-

A

Denaturation, Annealing, Synthesis

B

Synthesis, Annealing, Denaturation

C

Annealing, Synthesis, Denaturation

D

Denaturation, Synthesis, Annealing

Question 45: Palette 45/339

Allen MCQ

What is the source of the Ti (Tumor inducing) plasmid which is modified and used as a cloning vector to deliver the desirable genes into plant cells ?

A

Agrobacterium tumifaciens

B

Thermophilus aquaticus

C

Pyrococcus furiosus

D

Aedes aegypti

Question 46: Palette 46/339

Allen MCQ

The thermostable enzymes, 'Taq' isolated from thermophilic bacteria is :-

A

RNA polymerases

B

DNA polymerases

C

Restriction endonucleases

D

DNA ligases

Question 47: Palette 47/339

Allen MCQ

The term "molecular scissors" generally refers to:-

A

DNA polymerases

B

RNA polymerases

C

Restriction endonucleases

D

DNA ligases

Question 48: Palette 48/339

Allen MCQ

In the "PCR" technology the DNA segment is replicated over a billion times. This repeated replications catalyzed by the enzyme :-

A

RNA polymerase

B

Taq polymerase

C

DNA dependent RNA polymerase

D

Primase

Question 49: Palette 49/339

Allen MCQ

The restriction enzyme(s) used in recombinant DNA technology making staggered cuts in DNA leaving sticky ends is/are :-

A

EcoR I

B

Hind III

C

BamH I

D

All of the above

Question 50: Palette 50/339

Allen MCQ

Restriction enzyme Eco RI cuts the DNA between bases G and A only when the sequence in DNA is:-

A

GATATC

B

GAATTC

C

GATTCC

D

GAACTT

Question 51: Palette 51/339

Allen MCQ

'Transgenic' plants are produced by :-

A

Inducing gene mutation

B

Arresting spindle fibre formation

C

Deleting sex chromosomes

D

Introducing foreign genes

Question 52: Palette 52/339

Allen MCQ

For a DNA to function as a cloning vector the most essential requirement is :-

A

multiple restriction sites

B

several selectable markers

C

circular nature

D

'ori' sequence

Question 53: Palette 53/339

Allen MCQ

According to EFB, "The integration of natural science and organisms, cells, parts thereof and molecular analogues for products and services," is known as:-

A

Biochemistry

B

Bioinformatics

C

Biotechnology

D

Biology

Question 54: Palette 54/339

Allen MCQ

The stickiness of DNA ends facilitates the action of which enzyme :-

A

DNA polymerase

B

DNA Ligase

C

Restriction endonuclease

D

Alkaline phosphatase

Question 55: Palette 55/339

Allen MCQ

Which technique is used to check the progression of restriction enzyme digestion?

A

""

B

Gel electrophoresis

C

Southern Blotting

D

Staining

Question 56: Palette 56/339

Allen MCQ

In gel electrophoresis, at which end of the gel the sample is loaded?

A

In the wells

B

Towards positive electrode

C

Towards negative electrode

D

1 & 3 both

Question 57: Palette 57/339

Allen MCQ

An antibiotic resistance gene of plasmid vector which get inactivated due to insertion of alien DNA, helps in the selection of:-

A

Transformants

B

Recombinants

C

Non-Transformants

Question 58: Palette 58/339

Allen MCQ

In which type of bioreactor air bubbles dramatically increases the oxygen transfer area?

A

Simple stirred tank bioreactor

B

Sparged stirred tank bioreactor

C

Both (1) & (2)

D

None of these

Question 59: Palette 59/339

Allen MCQ

Genetic modification (GM) has been used to:-

A

Create tailor made plants

B

Supply alternative resources to industries

C

Enhanced nutritional value of food.

D

All of the above

Question 60: Palette 60/339

Allen MCQ

The choice of Bt-gene for experiment depends upon:-

A

The host plant/crop

B

Targeted pest/insect

C

Bacillus strain

D

1 & 2 both

Question 61: Palette 61/339

Allen MCQ

In nematode resistance by RNA interference, some specific genes were introduced which form dsRNA. These were introduced in:-

A

Nematode

B

Host plant

C

Agrobacterium

D

All of these

Question 62: Palette 62/339

Allen MCQ

Select the incorrect match:-

A

Transgenic mice - Polio vaccine

B

Rosie cow - $\alpha$-lactalbumin gene

C

ssDNA/RNA probe - Gene therapy

D

"PCR" - Molecular diagnosis

Question 63: Palette 63/339

Allen MCQ

In EcoR I, R is stand for :-

A

Strain

B

Species

C

Genus

D

Order

Question 64: Palette 64/339

Allen MCQ

Restriction endonucleases are used in genetic engineering to form:-

A

Recombinant molecule of protein

B

Recombinant molecule of DNA

C

Recombinant molecule of protein & DNA

D

Recombinant cell

Question 65: Palette 65/339

Allen MCQ

Which instrument is used for the separation of DNA fragments?

A

""

B

Gel electrophoresis

C

Bioreactor

D

Restriction endonuclease

Question 66: Palette 66/339

Allen MCQ

Which of following feature is not necessary for cloning vector?

A

Origin of replication

B

High copy number

C

Selectable marker

D

Cloning sites

Question 67: Palette 67/339

Allen MCQ

Which of the following is not true for cloning vector?

A

more than two origin site of replication

B

vector should have selectable marker gene

C

single recognition site for the commonly used restriction enzyme

D

pBR-322 have tetracycline resistance gene

Question 68: Palette 68/339

Allen MCQ

Transformation is a procedure through which:-

A

A piece of DNA is introduced in a host bacterium

B

A piece of DNA is introduced in a vector

C

A piece of DNA is introduced from protein

D

All of these

Question 69: Palette 69/339

Allen MCQ

Second letter of the name of restriction endonuclease came from the:-

A

Genus of organism

B

Species of organism

C

Family of organism

D

Class of organism

Question 70: Palette 70/339

Allen MCQ

To isolate DNA from fungi we have to break the wall. This is done by:-

A

Lysozyme

B

Cellulose

C

Invertase

D

Chitinase

Question 71: Palette 71/339

Allen MCQ

Which of the following enzyme will get inactivated in insertional inactivation?

A

Transacetylase

B

Permease

C

$\beta$-galactosidase

D

Taq-polymerase

Question 72: Palette 72/339

Allen MCQ

In presence of chromogenic substrate recombinant bacteria gives:-

A

Red coloured colonies

B

Colourless colonies

C

Blue colonies

D

Insulin

Question 73: Palette 73/339

Allen MCQ

Which of the following enzyme is known as molecular scissors?

A

Ligase

B

DNA polymerase

C

Restriction enzyme

D

Helicase

Question 74: Palette 74/339

Allen MCQ

Which of the following is not required in "PCR"?

A

DNA primer

B

DNA template

C

RNA primer

D

Taq polymerase

Question 75: Palette 75/339

Allen MCQ

The substrate for restriction enzyme is:-

A

Single stranded RNA

B

Proteins

C

Double stranded DNA

D

Single stranded DNA

Question 76: Palette 76/339

Allen MCQ

In recombinant DNA technology, the term vectors refers to:-

A

the enzyme that cuts DNA into restriction fragments

B

the sticky end of a DNA fragment

C

a plasmid used to transfer DNA into a living cell

D

a DNA probe used to identify a particular gene

Question 77: Palette 77/339

Allen MCQ

Which is frequently used as a vector for cloning gene is:-

A

an original bacterial plasmid

B

a modified bacterial plasmid

C

a viral genome

D

a transposon

Question 78: Palette 78/339

Allen MCQ

Genetically engineered bacteria have been used in commercial production of:-

A

Thyroxin

B

Testosterone

C

Human insulin

D

Melatonium

Question 79: Palette 79/339

Allen MCQ

Important objective of biotechnology in agriculture section is:-

A

To produce pest resistant varieties of plants

B

To increase the nitrogen content

C

To decrease the seed number

D

To increase the plant weight

Question 80: Palette 80/339

Allen MCQ

The name of drug used in cancer treatment produced by biotechnology is:-

A

Interferon

B

[HGH] Human growth hormone

C

TSH

D

Insulin

Question 81: Palette 81/339

Allen MCQ

The prerequisites for biotechnological production of antibiotics is :-

A

To search an antibiotic producing microorganism

B

To isolate the antibiotic gene

C

To join antibiotic gene with E.coli plasmid

D

All of the above

Question 82: Palette 82/339

Allen MCQ

Modern biotechnology consist :-

A

Genetic engineering

B

tissue culture

C

Microbiology

D

All the above

Question 83: Palette 83/339

Allen MCQ

First artificially synthesized hormone is :-

A

Secretin

B

Insulin

C

Glucagon

D

Renin

Question 84: Palette 84/339

Allen MCQ

The protein products of the following Bt toxin genes cryIAc and cryIIAb are responsible for controlling:-

A

Bollworm

B

Roundworm

C

Moth

D

Fruit fly

Question 85: Palette 85/339

Allen MCQ

A transgenic rice (Golden rice) has been developed for increased content of :-

A

Vitamin A

B

Vitamin $B_1$

C

Vitamin C

D

Vitamin D

Question 86: Palette 86/339

Allen MCQ

During the processing of the prohormone "proinsulin" into the mature "insulin" :-

A

C-peptide is added to proinsulin

B

C-peptide is removed from proinsulin

C

B-peptide is added to proinsulin

D

B-peptide is removed from proinsulin

Question 87: Palette 87/339

Allen MCQ

A genetically engineered bacteria used for clearing oil spills is :-

A

Escherischia coli

B

Bacillus subtilis

C

Agrobacterium tumifaciens

D

Pseudomonas putida

Question 88: Palette 88/339

Allen MCQ

First transgenic plant :-

A

Potato

B

Tomato

C

Tobacco

D

Maize

Question 89: Palette 89/339

Allen MCQ

E. coli are used in production of :-

A

Rifampicin

B

LH

C

Ecdyson

D

Interferon

Question 90: Palette 90/339

Allen MCQ

A giant rat is formed in the laboratory, what is the reason :-

A

Gene mutation

B

Gene synthesis

C

Gene manipulation

D

Gene replication

Question 91: Palette 91/339

Allen MCQ

Cultivation of Bt cotton has been much in the news. The prefix ‘‘Bt’’ means :-

A

‘‘Barium – treated’’ cotton seeds.

B

‘‘Bigger thread’’ variety of cotton with batter tensile strength.

C

Produced by ‘‘biotechnology’’ using restriction enzymes and ligases.

D

Carrying an endotoxin gene from Bacillus thuringiensis.

Question 92: Palette 92/339

Allen MCQ

cry-gene which synthesize crystal protein isolated from :-

A

Bacillus thuriengensis

B

Rhizobium

C

Bacillus polymyxa

D

Clostridium

Question 93: Palette 93/339

Allen MCQ

Which of the following combination of risk are associated with genetically modified food?

A

Toxicity

B

Allergic reaction

C

Antibiotic resistance in microorganism present in alimentary canal

D

All the above

Question 94: Palette 94/339

Allen MCQ

Gene therapy first used in the treatment of:-

A

Albinism

B

Haemophilia

C

SCID

D

LIQID

Question 95: Palette 95/339

Allen MCQ

Bt–cotton is resistant for :-

A

Round–worm

B

Fluke–worm

C

Boll–worm

D

Pin–worm

Question 96: Palette 96/339

Allen MCQ

Genetically engineered human insulin is called :-

A

Humulin

B

Haematin

C

Hybridoma

D

Hybrid

Question 97: Palette 97/339

Allen MCQ

The C-peptide is :-

A

not present in proinsulin

B

present in mature insulin

C

removed during maturation of insulin

D

also present in artificial insulin

Question 98: Palette 98/339

Allen MCQ

GEAC makes decisions regarding:-

A

the validity of GM research

B

the safety of introducing GM organisms for public services

C

the validity of biopatents

D

more than one options are correct

Question 99: Palette 99/339

Allen MCQ

The Indian parliament has recently cleared which amendment of the Indian patents bill:-

A

1st

B

2nd

C

3rd

D

4th

Question 100: Palette 100/339

Allen MCQ

The use of bio-resources by multinational companies & other organisations without proper authorisation from the countries & people concerned, is known as:-

A

Biopatent

B

Biopiracy

C

Biowar

D

Biodiversity

Question 101: Palette 101/339

Allen MCQ

Which of the following peptide chain in not present in mature insulin?

A

A-peptide

B

B-peptide

C

C-peptide

D

A & B peptide

Question 102: Palette 102/339

Allen MCQ

Two microbes found to be very useful in genetic engineering are :–

A

Escherichia coli and Agrobacterium tumefaciens

B

Vibrio cholerae and a tailed bacteriophage

C

Diplococcus sp. and Pseudomonas sp.

D

Crown gall bacterium and Caenorhabditis elegans

Question 103: Palette 103/339

Allen MCQ

Restriction endonuclease :–

A

Cuts the DNA molecule randomly

B

Cuts the DNA molecule at specific sites

C

Restricts the synthesis of DNA inside the nucleus

D

Synthesizes DNA

Question 104: Palette 104/339

Allen MCQ

Golden rice is a promising transgenic crop. When released for cultivation, it will help in:-

A

Alleviation of vitamin A deficiency

B

Pest resistance

C

Herbicide tolerance

D

Producing a petrol-like fuel from rice

Question 105: Palette 105/339

Allen MCQ

A genetically engineered micro-organism used successfully in bioremediation of oil spills is a species of :-

A

Pseudomonas

B

Trichoderma

C

Xanthomonas

D

Bacillus

Question 106: Palette 106/339

Allen MCQ

Cry1 endotoxins obtained from Bacillus thuringiensis are effective against :-

A

Flies

B

Nematodes

C

Boll worms

D

Mosquitoes

Question 107: Palette 107/339

Allen MCQ

Human insulin is being commercially produced from a transgenic species of :-

A

Mycobacterium

B

Rhizobium

C

Saccharomyces

D

Escherichia

Question 108: Palette 108/339

Allen MCQ

Main objective of production/use of herbicide resistant GM crops is to :-

A

Eliminate weeds from the field without the use of herbicides

B

Encourage eco-friendly herbicides

C

Reduce herbicide accumulation in food articles for health safety

D

Eliminate weeds from the field without the use of manual labour

Question 109: Palette 109/339

Allen MCQ

A transgenic food crop which may help in solving the problem of night blindness in developing countries is :-

A

Starlink maize

B

Bt Soybean

C

Golden rice

D

Flavr Savr tomatoes

Question 110: Palette 110/339

Allen MCQ

Which one of the following is commonly used in transfer of foreign DNA into crop plants ?

A

Penicillium expansum

B

Trichoderma harzianum

C

Meloidogyme incognita

D

Agrobacterium tumefaciens

Question 111: Palette 111/339

Allen MCQ

Polyethylene glycol method is used for :-

A

Energy production from sewage

B

Gene transfer without a vector

C

Biodiesel production

D

Seedless fruit production

Question 112: Palette 112/339

Allen MCQ

Transgenic plants are the ones :-

A

Grown in artificial medium after
hybridization in the field

B

Produced by a somatic embryo in artificial
medium

C

Generated by introducing foreign DNA in
to a cell and regenerating a plant from
that cell

D

Produced after protoplast fusion in
artificial medium

Question 113: Palette 113/339

Allen MCQ

The bacterium Bacillus thuringiensis is widely used in contemporary biology as :-

A

Source of industrial enzyme

B

Indicator of water pollution

C

Insecticide

D

Agent for production of dairy products

Question 114: Palette 114/339

Allen MCQ

What is true about Bt toxin ?

A

The concerned Bacillus has antitoxins

B

The inactive protoxin gets converted into
active form in the insect gut

C

Bt protein exists as active toxin in the
Bacillus

D

The activated toxin enters the ovaries of
the pest to sterilise it and thus prevent its
multiplication

Question 115: Palette 115/339

Allen MCQ

The genetic defect adenosine deaminase (ADA) deficiency may be cured permanently by:

A

Enzyme replacement therapy

B

Periodic infusion of genetically engineered lymphocytes having functional ADA cDNA

C

Administering adenosine deaminase activators

D

Introducing bone marrow cells producing ADA into cells at early embryonic stages

Question 116: Palette 116/339

Allen MCQ

Which one of the following is used as vector for cloning genes into higher organisms?

A

Rhizopus nigricans

B

Retrovirus

C

Baculovirus

D

Salmonella typhimurium

Question 117: Palette 117/339

Allen MCQ

Restriction endonucleases are enzymes which :-

A

restrict the action of the enzyme DNA polymerase

B

remove nucleotides from the ends of the DNA molecule

C

make cuts at specific positions within the DNA molecule

D

recognize a specific nucleotide sequence for binding of DNA ligase

Question 118: Palette 118/339

Allen MCQ

Which one of the following palindromic base sequences in DNA can be easily cut at about the middle by some particular restriction enzyme?

A

5' ____GAATTC ______3' 3' CTTAAG _______5'

B

5' ____CACGTA ____3' 3' CTCAGT ________5'

C

5' ________CGTTCG ______3' 3' ATGGTA __________5'

D

5' ________GATATG ______3' 3' CTACTA _____5'

Question 119: Palette 119/339

Allen MCQ

DNA or RNA segment tagged with a radiactive molecule is called:-

A

Clone

B

Plasmid

C

Vector

D

Probe

Question 120: Palette 120/339

Allen MCQ

An improved variety of transgenic basmati rice :-

A

is completely resistant to all insect pests and diseases of paddy

B

gives high yield but has no charactristic aroma

C

does not require chemical fertilizers and growth hormones

D

give high yield and is rich in vitamin A

Question 121: Palette 121/339

Allen MCQ

Genetic engineering has been successfuly used for producing :-

A

transgenic Cow-Roise which produces high fat milk for making ghee

B

animals like bulls for farm work as they have super power

C

transgenic mice for testing safety of polio vaccine before use in humans

D

transgenic models for studying new treatments for certain cardiac diseases

Question 122: Palette 122/339

Allen MCQ

The genetically-modified (GM) brinjal in India has been developed for :-

A

Enhancing mineral content

B

Drought-resistance

C

Insect-resistance

D

Enhancing shelf life

Question 123: Palette 123/339

Allen MCQ

Some of the characteristics of Bt cotton are:-

A

High yield and production of toxic protein crystals which kill dipteran pests

B

High yield and resistance to bollworms

C

Long fibre and resistance to aphids

D

Medium yield, long fibre and resistance to beetle pests

Question 124: Palette 124/339

Allen MCQ

Given below is a sample of a portion of DNA strand giving the base sequence on the opposite strands. What is so special shown in it? 5'______________GAATTC__________3' 3'________CTTAAG__________5'

A

Replication completed

B

Delection mutation

C

Start codon at the 5' end

D

Palindromic sequence of base pairs

Question 125: Palette 125/339

Allen MCQ

There is a restriction endonuclease called Eco RI. What does "co" part in it stand for ?

A

Colon

B

Coelom

C

Coenzyme

D

Coli

Question 126: Palette 126/339

Allen MCQ

Agarose extracted from sea weeds finds use in:

A

Spectrophotometry

B

Tissue Culture

C

"PCR"

D

Gel electrophoresis

Question 127: Palette 127/339

Allen MCQ

Maximum number of existing transgenic animals is of :-

A

Fish

B

Mice

C

Cow

D

Pig

Question 128: Palette 128/339

Allen MCQ

The process of RNA interference has been used in the development of plants resistant to:

A

Nematodes

B

Fungi

C

Viruses

D

Insects

Question 129: Palette 129/339

Allen MCQ

Read the following four statements (A-D) about certain mistakes in two of them:-
(A) The first transgenic buffalo, Rosie produced milk which was human alphalactalbumin enriched. (B) Restriction enzymes are used in isolation of DNA from other macro molecules. (C) Downstream processing is one of the steps of R-DNA technology. (D) Disarmed pathogen vectors are also used in transfer of R-DNA into the host. Which are the two statements having mistakes ?

A

Statements A and B

B

Statements B and C

C

Statements C and D

D

StatementsA and C

Question 130: Palette 130/339

Allen MCQ

Silencing of mRNA has been used in producing transgenic plants resistant to:-

A

Bacterial blights

B

Bollworms

C

Nematodes

D

White rusts

Question 131: Palette 131/339

Allen MCQ

Bacillus thuringiensis forms protein crystals which contain insecticidal protein. This protein :-

A

does not kill the carrier bacterium which is itself resistant to this toxin

B

binds with epithelial cells of midgut of the insect pest ultimately killing it

C

is coded by several genes including the gene cry

D

is activated by acid pH of the foregut of
ect pest

Question 132: Palette 132/339

Allen MCQ

Which one of the following techniques made it possible to genetically engineer living organisms?

A

Hybridization

B

Recombinant DNA techniques

C

X-ray diffraction

D

Heavier isotope labelling

Question 133: Palette 133/339

Allen MCQ

Which one is a true statement regarding DNA polymerase used in "PCR" ?

A

It is isolated from a virus

B

It remains active at high temperature

C

It is used to ligate introduced DNA in recipient cells

D

It serves as a selectable marker

Question 134: Palette 134/339

Allen MCQ

For transformation, micro-particles coated with DNA to be bombarded with gene gun are made up of :-

A

Silicon or Platinum

B

Gold or Tungsten

C

Silver or platinum

D

Platinum or zinc

Question 135: Palette 135/339

Allen MCQ

The figure below is the diagrammatic representation of the E. coli vector pBR322. Which one of the given options correctly identifies its certain component(s)?

A

Hind III, Eco RI-selectable markers

B

ampR, tetR-antibiotic resistance genes

C

ori-original restriction enzyme

D

rop-reduced osmotic pressure

Question 136: Palette 136/339

Allen MCQ

Consumption of which one of the following foods can prevent the kind of blindness associated with vitamin 'A' deficiency?

A

Golden rice

B

Bt-Brinjal

C

Flaver Savr 'tomato

D

Canolla

Question 137: Palette 137/339

Allen MCQ

DNA or RNA segment tagged with a radiactive molecule is called :-

A

Clone

B

Plasmid

C

Vector

D

Probe

Question 138: Palette 138/339

Allen MCQ

Which one of the following represents a palindromic sequence in DNA ?

A

$5 ^ { \prime } - \mathrm { C A T T A G } - 3 ^ { \prime }$ $5 ^ { \prime } - \mathrm { G A T A C C } - 3 ^ { \prime }$

B

$3 ^ { \prime } - \mathsf { G A T A A C } - 5 ^ { \prime }$ $3 ^ { \prime } { \cdot } \operatorname { C C T A A G } - 5 ^ { \prime }$

C

$5 ^ { \prime } - { \mathrm { G A A T T C } } - 3 ^ { \prime }$ $5 ^ { \prime } - \mathsf { C C A A T G } - 3 ^ { \prime }$

D

$3 ^ { \prime } { \cdot } \mathsf { C T T A A G } { \cdot } 5 ^ { \prime }$ $3 ^ { \prime } - \mathrm { G A A T C C } - 5 ^ { \prime }$

Question 139: Palette 139/339

Allen MCQ

In genetic engineering, the antibiotics are used :-

A

as sequences from where replication starts

B

to keep the cultures free of infection

C

as selectable markers

D

to select healthy vectors

Question 140: Palette 140/339

Allen MCQ

The figure below shows three steps (A,B, C) of Polymerase Chain Reaction ("PCR"). Select the option giving correct identification together with what it represents?

A

C-Extension in the presence of heat stable DNA polymerase

B

A-Annealing with two sets of primers

C

B-Denaturation at a temperature of about 98°C separating the two DNA strands

D

A-Denaturation at a temperature of about $5 0 ^ { \circ } \mathrm { C }$

Question 141: Palette 141/339

Allen MCQ

BioColumnics (gene-gun) is suitable for :-

A

Constructing recombinant DNA by joining with vectors

B

DNA finger printing

C

Disarming pathogen vectors

D

Transformation of plants cells

Question 142: Palette 142/339

Allen MCQ

Tobacco plants resistant to a nematode have been developed by the introduction of DNA that produced (in the host cells) :-

A

an antifeedant

B

a toxic protein

C

both sense and anti-sense RNA

D

a particular hormone

Question 143: Palette 143/339

Allen MCQ

The first clinical gene therapy was given for treating :-

A

Rheumatoid arthritis

B

Adenosine deaminase deficiency

C

Diabetes mellitus

D

Chicken pox

Question 144: Palette 144/339

Allen MCQ

The colonies of recombinant bacteria appear white in contrast to blue colonies of nonrecombinant bacteria because of :-

A

Inactivation of glycosidase enzyme in recombinant bacteria

B

Non-recombinant bacteria containing beta-galactosidase

C

Insertional inactivation of alphagalactosidase in non-recombinant bacteria

D

Insertional inactivation of betagalactosidase in recombinant bacteria

Question 145: Palette 145/339

Allen MCQ

DNA fragments generated by the restriction endonucleases in a chemical reaction can be separated by :-

A

Restriction mapping

B

Centrifugation

C

Polymerase chain reaction

D

Electrophoresis

Question 146: Palette 146/339

Allen MCQ

An analysis of chromosomal DNA using the Southern hybridization technique does not use:

A

Electrophoresis

B

Blotting

C

Autoradiography

D

"PCR"

Question 147: Palette 147/339

Allen MCQ

Which vector can clone only a small fragment of DNA?

A

Bacterial artificial chromosome

B

Yeast artificial chromosome

C

Plasmid

D

Cosmid

Question 148: Palette 148/339

Allen MCQ

The first human hormone produced by recombinant DNA technology is :-

A

Insulin

B

Estrogen

C

Thyroxin

D

Progesterone

Question 149: Palette 149/339

Allen MCQ

The crops engineered for glyphosate are resistant/tolerant to :-

A

Bacteria

B

Insects

C

Herbicides

D

Fungi

Question 150: Palette 150/339

Allen MCQ

In Bt cotton, the Bt toxin present in plant tissue as pro-toxin is converted into active toxin due to:-

A

Acidic pH of the insect gut

B

Action of gut micro-organisms

C

Presence of conversion factors in insect gut

D

Alkaline pH of the insect gut

Question 151: Palette 151/339

Allen MCQ

Which body of the Government of India regulates GM research and safety of introducing GM organisms for public services?

A

Indian Council of Agricultural Research

B

Genetic Engineering Approval Committee

C

Research Committee on Genetic Manipulation

D

Bio-safety committee

Question 152: Palette 152/339

Allen MCQ

The DNA molecules to which the gene of interest is integrated for cloning is called :-

A

Carrier

B

Transformer

C

Vector

D

Template

Question 153: Palette 153/339

Allen MCQ

The cutting of DNA at specific locations became possible with the discovery of :-

A

Ligases

B

Restriction enzymes

C

Probes

D

Selectable markers

Question 154: Palette 154/339

Allen MCQ

Golden rice is a genetically modified crop plant where the incorporated gene is meant for biosynthesis of :-

A

Vitamin A

B

Vitamin B

C

Vitamin C

D

Omega 3

Question 155: Palette 155/339

Allen MCQ

The introduction of T-DNA into plants involves: -

A

Allowing the plant roots to stand in water

B

Infection of the plant by Agrobacterium tumefaciens

C

Altering the pH of the soil, then heat shocking the plants

D

Exposing the plants to cold for a brief period

Question 156: Palette 156/339

Allen MCQ

The two polypeptides of human insulin are linked together by :-

A

Hydrogen bonds

B

Phosphodiester bond

C

Covalent bond

D

Disulphide bridges

Question 157: Palette 157/339

Allen MCQ

Which of the following is not a feature of the plasmids ?

A

Independent replication

B

Circular structure

C

Transferable

D

Single - stranded

Question 158: Palette 158/339

Allen MCQ

The taq polymerase enzyme is obtained from:-

A

Thermus aquaticus

B

Thiobacillus ferroxidans

C

Bacillus subtilis

D

Pseudomonas putida

Question 159: Palette 159/339

Allen MCQ

Which of the following is a restriction endonuclease?

A

Hind II

B

Protease

C

DNase I

D

RNase

Question 160: Palette 160/339

Allen MCQ

Stirred-tank bioreactors have been designed for :-

A

availability of oxygen throughout the process

B

ensuring anaerobic conditions in the culture vessel

C

purification of product

D

addition of preservatives to the product

Question 161: Palette 161/339

Allen MCQ

A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using :-

A

Polymerase-III

B

Ligase

C

Eco RI

D

Taq polymerase

Question 162: Palette 162/339

Allen MCQ

Which of the following is not a component of downstream processing ?

A

Preservation

B

Expression

C

Separation

D

Purification

Question 163: Palette 163/339

Allen MCQ

Which of the following restriction enzymes produces blunt ends ?

A

Xho I

B

Hind III

C

Sal I

D

Eco RV

Question 164: Palette 164/339

Allen MCQ

The DNA fragments separated on an agarose gel can be visualised after staining with :-

A

Acetocarmine

B

Aniline blue

C

Ethidium bromide

D

Bromophenol blue

Question 165: Palette 165/339

Allen MCQ

The process of separation and purification of expressed protein before marketing is called:

A

Downstream processing

B

Bioprocessing

C

Postproduction processing

D

Upstream processing

Question 166: Palette 166/339

Allen MCQ

A gene whose expression helps to identify transformed cell is known as :-

A

Vector

B

Plasmid

C

Structural gene

D

Selectable marker

Question 167: Palette 167/339

Allen MCQ

Which of the following is commonly used as a vector for introducing a DNA fragment in human lymphocytes ?

A

Retrovirus

B

Ti plasmid

C

$\lambda phage$

D

pBR322

Question 168: Palette 168/339

Allen MCQ

In India, the organisation responsible for assessing the safety of introducing genetically modified organisms for public use is :-

A

Indian Council of Medical Research (ICMR)

B

Council for Scientific and Industrial Research (CSIR)

C

Research Committee on Genetic Manipulation (RCGM)

D

Genetic Engineering Appraisal Committee (GEAC)

Question 169: Palette 169/339

Allen MCQ

A 'new variety of rice was patented by a foreign company though such varieties have been present in India for a long time. This is related to :-

A

Co-667

B

Sharbati Sonora

C

Lerma Rojo

D

Basmati

Question 170: Palette 170/339

Allen MCQ

Use of bioresources by multinational companies and organisations without authorisation from the concerned country and its people is called :-

A

Bio-infringement

B

Biopiracy

C

Biodegradation

D

Bioexploitation

Question 171: Palette 171/339

Allen MCQ

The correct order of steps in Polymerase Chain Reaction ("PCR") is :-

A

Extension, Denaturation, Annealing

B

Annealing, Extension, Denaturation

C

Denaturation, Extension, Annealing

D

Denaturation, Annealing, Extension

Question 172: Palette 172/339

Allen MCQ

Which of the following is true for Golden rice?

A

It is Vitamin A enriched, with a gene from daffodil

B

It is pest resistant, with a gene from Bacillus thuringiensis

C

It is drought tolerant, developed using Agrobacterium vector

D

It has yellow grains, because of a gene introduced from a primitive variety of rice

Question 173: Palette 173/339

Allen MCQ

Which of the following features of genetic code does allow bacteria to produce human insulin by recombinant DNA technology?

A

Genetic code is not ambiguous

B

Genetic code is redundant

C

Genetic code is nearly universal

D

Genetic code is specific

Question 174: Palette 174/339

Allen MCQ

Following statements describe the characteristics of the enzyme Restriction endonuclease. Identify the incorrect statement:

A

The enzyme cuts DNA molecule at identified position within the DNA

B

The enzyme binds DNA at specific sites and cuts only one of the two strands.

C

The enzyme cuts the sugar-phosphate backbone at specific sites on each strand.

D

The enzyme recognizes a specific palindromic nucleotide sequence in the DNA

Question 175: Palette 175/339

Allen MCQ

DNA precipitation out of a mixture of biomolecules can be achieved by treatment with :-

A

Isopropanol

B

Chilled ethanol

C

Methanol at room temperature

D

Chilled chloroform

Question 176: Palette 176/339

Allen MCQ

What triggers activation of protoxin to active Bt toxin of Bacillus thuringiensis in boll worm?

A

Body temperature

B

Moist surface of midgut

C

Alkaline pH of gut

D

Acidic pH of stomach

Question 177: Palette 177/339

Allen Match the Columns

Match the following enzymes with their functions:-
Match Column-I with Column-II

Column-I	Column-II
(A) Restriction endonuclease (B) Restriction exonuclease (C) DNA ligase (D) Taq polymerase	(I) Joins the DNA fragements (II) Extends primers on genomic DNA template (III) Cuts DNA at specific position (IV) Removes nucleotides from the ends of DNA

A

A-III, B-I, C-IV, D-II

B

A-III, B-IV, C-I, D-II

C

A-IV, B-III, C-I, D-II

D

A-II, B-IV, C-I, D-III

Question 178: Palette 178/339

Allen MCQ

The two antibiotic resistance genes on vector pBR322 are :-

A

Ampicillin and Tetracycline

B

Ampicillin and Chloramphenicol

C

Chloramphenicol and Tetracycline

D

Tetracycline and Kanamycin

Question 179: Palette 179/339

Allen MCQ

Exploitation of bioresources of a nation by multinational companies without authorization from the concerned country is referred to as: -

A

Bioweapon

B

Biopiracy

C

Bioethics

D

Biowar

Question 180: Palette 180/339

Allen MCQ

A selectable marker is used to: -

A

help in eliminating the non-transformants, so that the transformants can be regenerated

B

identify the gene for a desired trait in an alien organism

C

select a suitable vector for transformation in a specific crop

D

mark a gene on a chromosome for isolation using restriction enzyme

Question 181: Palette 181/339

Allen MCQ

Given below are four statements pertaining to separation of DNA fragments using gel electrophoresis. Identify the incorrect statements: -
(a) DNA is negatively charged molecule and so it is loaded on gel towards the Anode terminal (b) DNA fragments travel along the surface of the gel whose concentration does not affect movement of DNA. (c) Smaller the size of DNA fragment larger is the distance it travels through it. (d) Pure DNA can be visualized directly by exposing UV radiation. Choose correct answer from the options given below

A

(a), (c) and (d)

B

(a), (b) and (c)

C

(b), (c) and (d)

D

(a), (b) and (d)

Question 182: Palette 182/339

Allen MCQ

An enzyme catalysing the removal of nucleotides from ends of DNA is:-

A

DNA ligase

B

Endonuclease

C

Exonuclease

D

Protease

Question 183: Palette 183/339

Allen MCQ

In RNAi, the genes are silenced using:-

A

ds-RNA

B

ss-DNA

C

ss-RNA

D

ds-DNA

Question 184: Palette 184/339

Allen MCQ

Which of the following statements is not correct?

A

Genetically engineered insulin is produced in E coli.

B

In man insulin is synthesised as a proinsulin.

C

The proinsulin has an extra peptide called C-peptide.

D

The functional insulin has A and B chains linked together by hydrogen bonds.

Question 185: Palette 185/339

Allen Match the Columns

Match the organism with its use in biotechnology.
Match Column-I with Column-II:

Column-I	Column-II
(A) Bacillus thuringiensis (B) Thermus aquaticus (C) Agrobacterium tumefaciens (D) Salmonella typhimurium	(I) Cloning vector (II) Construction of first rDNA molecule (III) DNA polymerase (IV) Cry proteins

A

A-III, B-IV, C-I, D-II

B

A-II, B-IV, C-III, D-I

C

A-IV, B-III, C-I, D-II

D

A-III, B-II, C-IV, D-I

Question 186: Palette 186/339

Allen MCQ

Bt cotton variety that was developed by the introduction of toxin gene of Bacillus thuringiensis (Bt) is resistant to :-

A

Insect predators

B

Insect pests

C

Fungal diseases

D

Plant nematodes

Question 187: Palette 187/339

Allen MCQ

The specific palindromic sequence which is recognized by EcoR I is :-

A

5' - GGATCC - 3' , 3' - CCTAGG - 5'

B

5' - GAATTC - 3' , 3' - CTTAAG - 5'

C

5' - GGAACC - 3' , 3' - CCTTGG - 5'

D

5' - CTTAAG - 3', 3' - GAATTC - 5'

Question 188: Palette 188/339

Allen MCQ

Choose the correct pair from the following:-

A

Exonucleases: - Make cuts at specific positions within DNA

B

Ligases :- Join the two DNA molecules

C

Polymerases: Break the DNA into fragments

D

Nucleases : Separate the two strands of DNA

Question 189: Palette 189/339

Allen Match the Columns

Match the following columns and select the correct
Match Column-I with Column-II:

Column-I	Column-II
(A) Bt cotton (B) Adenosine deaminase (C) RNAi (D) "PCR"	(I) Gene therapy (II) Cellular defence deficiency (III) Detection of HIV infection (IV) Bacillus thuringiensis

A

A-I, B-II, C-III, D-IV

B

A-IV, B-I, C-II, D-III

C

A-III, B-II, C-I, D-IV

D

A-II, B-III, C-IV, D-I

Question 190: Palette 190/339

Allen MCQ

Identify the wrong statement with regard to Restriction Enzymes.

A

Sticky ends can be joined by using DNA ligases.

B

Each restriction enzyme functions by inspecting the length of a DNA sequence.

C

They cut the strand of DNA at palindromic sites.

D

They are useful in genetic engineering.

Question 191: Palette 191/339

Allen MCQ

The sequence that controls the copy number of the linked DNA in the vector, is termed :-

A

Recognition site

B

Selectable marker

C

Ori site

D

Palindromic sequence

Question 192: Palette 192/339

Allen MCQ

First discovered restriction endonuclease that always cuts DNA molecule at a particular point by recognising a specific sequence of six base pairs is:-

A

EcoR1

B

Adenosine deaminase

C

Thermostable DNA polymerase

D

Hind II

Question 193: Palette 193/339

Allen MCQ

In Recombinant DNA technology antibiotics are used :-

A

to keep medium bacteria-free

B

to detect alien DNA

C

to impart disease-resistance to the host plant

D

as selectable markers

Question 194: Palette 194/339

Allen Match the Columns

Match the following techniques or instruments with their usage :- Match Column-I with Column-II:

Column-I	Column-II
(A) Bioreactor (B) Electrophoresis (C) "PCR" (D) ELISA	(I) Separation of DNA fragments (II) Production of large quantities of products (III) Detection of pathogen, based on antigen – antibody reaction (IV) Amplification of nucleic acids

A

A-III, B-II, C-IV, D-I

B

A-II, B-I, C-IV, D-III

C

A-IV, B-III, C-II, D-I

D

A-II, B-I, C-III, D-IV

Question 195: Palette 195/339

Allen MCQ

In a mixture, DNA fragments are separated by:-

A

Bioprocess engineering

B

Restriction digestion

C

Electrophoresis

D

Polymerase chain reaction

Question 196: Palette 196/339

Allen MCQ

Spooling is :-

B

Cutting of separated DNA bands from the agarose gel

C

Transfer of separated DNA fragments to synthetic membranes

D

Collection of isolated DNA

Question 197: Palette 197/339

Allen MCQ

Select the correct statement from the following :-

A

Gel electrophoresis is used for amplification of a DNA segment.

B

The polymerase enzyme joins the gene of interest and the vector DNA.

C

Restriction enzyme digestions are performed by incubating purified DNA molecules with the restriction enzymes of optimum conditions.

D

"" is used for isolation and separation of gene of interest.

Question 198: Palette 198/339

Allen MCQ

RNA interference is used for which of the following purposes in the field of biotechnology ?

A

to develop a plant tolerant to abiotic stresses

B

to develop a pest resistant plant against infestation by nematode

C

to enhance the mineral usage by the plant

D

to reduce post-harvest losses

Question 199: Palette 199/339

Allen MCQ

The laws and rules to prevent unauthorised exploitation of bio-resources are termed as-

A

Biopatenting

B

Bioethics

C

Bioengineering

D

Biopiracy

Question 200: Palette 200/339

Allen MCQ

During the purification process for recombinant DNA technology, addition of chilled ethanol precipitates out:-

A

RNA

B

DNA

C

Histones

D

Polysaccharides

Question 201: Palette 201/339

Allen MCQ

Which of the following is a correct sequence of steps in a "PCR" (Polymerase Chain Reaction) ?

A

Denaturation, Annealing, Extension

B

Denaturation, Extension, Annealing

C

Extension, Denaturation, Annealing

D

Annealing, Denaturation, Extension

Question 202: Palette 202/339

Allen MCQ

DNA strands on a gel stained with ethidium bromide when viewed under UV radiation, appear as :-

A

Yellow bands

B

Bright orange bands

C

Dark red bands

D

Bright blue bands

Question 203: Palette 203/339

Allen MCQ

When gene targetting involving gene amplification is attempted in an individual's tissue to treat disease, it is known as :-

A

Biopiracy

B

Gene therapy

C

Molecular diagnosis

D

Safety testing

Question 204: Palette 204/339

Allen MCQ

Which of the following is not an application of "PCR" (Polymerase Chain Reaction) ?

A

Molecular diagnosis

B

Gene amplification

C

Purification of isolated protein

D

Detection of gene mutation

Question 205: Palette 205/339

Allen MCQ

Plasmid pBR322 has PstI restriction enzyme site within gene ampR that confers ampicillin resistance. If this enzyme is used for inserting a gene for β-galactoside production and the recombinant plasmid is inserted in an E.coli strain

A

it will not be able to confer ampicillin resistance to the host cell.

B

the transformed cells will have the ability to resist ampicillin as well as produce β- galactoside.

C

it will lead to lysis of host cell.

D

it will be able to produce a novel protein with dual ability.

Question 206: Palette 206/339

Allen MCQ

A specific recognition sequence identified by endonucleases to make cuts at specific positions within the DNA is :-

A

Degenerate primer sequence

B

Okazaki sequences

C

Palindromic Nucleotide sequences

D

Poly(A) tail sequences

Question 207: Palette 207/339

Allen MCQ

With regard to insulin choose correct options.
(a) C-peptide is not present in mature insulin.
(b) The insulin produced by rDNA technology has C-peptide.
(c) The pro-insulin has C-peptide.
(d) A-peptide and B-peptide of insulin are interconnected by disulphide bridges.
Choose the correct answer from the options given below.

A

(b) and (d) only

B

(b) and (c) only

C

(a), (c) and (d) only

D

(a) and (d) only

Question 208: Palette 208/339

Allen MCQ

During the process of gene amplification using "PCR", if very high temperature is not maintained in the beginning, then which of the following steps of "PCR" will be affected first?

A

Annealing

B

Extension

C

Denaturation

D

Ligation

Question 209: Palette 209/339

Allen MCQ

For effective treatment of the disease, early diagnosis and understanding its pathophysiology is very important. Which of the following molecular diagnostic techniques is very useful for early detection?

A

Western Blotting Technique

B

Southern Blotting Technique

C

ELISA Technique

D

Hybridization Technique

Question 210: Palette 210/339

Allen MCQ

Which one of the following statement is not true regarding gel electrophoresis technique?

A

The separated DNA fragments are stained by using ethidium bromide.

B

The presence of chromogenic substrate gives blue coloured DNA bands on the gel.

C

Bright orange coloured bands of DNA can be observed in the gel when exposed to UV light.

D

The process of extraction of separated DNA strands from gel is called elution.

Question 211: Palette 211/339

Allen MCQ

Given below are two statements :-
one is labelled as Assertion (A) and the other is labelled as Reason (R).
Assertion (A) : Polymerase chain reaction is used in DNA amplification
Reason (R) : The ampicillin resistant gene is used as a selectable marker to check transformation.
In the light of the above statements,
choose the correct answer from the options given below:-

A

Both (A) and (R) are correct but (R) is not the correct explanation of (A)

B

(A) is correct but (R) is not correct

C

(A) is not correct but (R) is correct

D

Both (A) and (R) are correct and (R) is the correct explanation of (A)

Question 212: Palette 212/339

Allen MCQ

In the following palindromic base sequences of DNA, which one can be cut easily by particular restriction enzyme ?

A

5' G A A T T C 3'; 3' C T T A A G 5'

B

5' C T C A G T 3'; 3' G A G T C A 5'

C

5' G T A T T C 3'; 3' C A T A A G 5'

D

5' G A T A C T 3'; 3' C T A T G A 5'

Question 213: Palette 213/339

Allen MCQ

Given below are two statements:- Statement I : Restriction endonucleases recognise specific sequence to cut DNA known as palindromic nucleotide sequence. Statement II : Restriction endonucleases cut the DNA strand a little away from the centre of the palindromic site. In the light of the above statements, choose the most appropriate answer from the options given below:-

A

Both Statement I and Statement II are incorrect

B

Statement I is correct but Statement II is incorrect

C

Statement I is incorrect but Statement II is correct

D

Both Statement I and Statement II are correct

Question 214: Palette 214/339

Allen MCQ

Which of the following is not a desirable feature of a cloning vector ?

A

Presence of a marker gene

B

Presence of single restriction enzyme site

C

Presence of two or more recognition sites

D

Presence of origin of replication

Question 215: Palette 215/339

Allen MCQ

Transposons can be used during which one of the following?

A

Gene silencing

B

Autoradiography

C

Gene sequencing

D

Polymerase Chain Reaction

Question 216: Palette 216/339

Allen MCQ

Statements related to human Insulin are given below :-
Which statement(s) is/ are correct about genetically engineered Insulin ?
(a) Pro-hormone insulin contain extra stretch of C-peptide
(b) A-peptide and B-peptide chains of insulin were produced separately in E.coli, extracted and combined by creating disulphide bond between them.
(c) Insulin used for treating Diabetes was extracted from Cattles and Pigs.
(d) Pro-hormone Insulin needs to be processed for converting into a mature and functional hormone.
(e) Some patients develop allergic reactions to the foreign insulin.
Choose the most appropriate answer from the options given below:-

A

(b)only

B

(c) and (d) only

C

(c), (d) and (e) only

D

(a), (b) and (d) only

Question 217: Palette 217/339

Allen MCQ

Separation of DNA, fragments is done by a technique known as :-

A

Polymerase Chain Reaction

B

Recombinant technology

C

Southern blotting

D

Gel electrophoresis

Question 218: Palette 218/339

Allen Match the Columns

Match Column-I with Column-II :- Column-I Column-II

Column-I	Column-II
(A) Gene gun (B) Gene therapy (C) Gene cloning (D) Genome	(I) Replacement of a faulty gene by a normal healthy gene (II) Used for transfer of gene (III) Total DNA in the cells of an organism (IV) To obtain identical copies of a particular DNA molecule

A

A-II, B-I, C-IV, D-III

B

A-I, B-III, C-II, D-IV

C

A-IV, B-I, C-III, D-II

D

A-II, B-III, C-IV, D-I

Question 219: Palette 219/339

Allen MCQ

Pathogenic bacteria gain resistance to antibiotics due to changes in their :-

A

Cosmids

B

Plasmids

C

Nucleus

D

Nucleoid

Question 220: Palette 220/339

Allen MCQ

Which of the following methods is not commonly used for introducing foreign DNA into the plant cell?

A

Agrobacterium mediated transformation

B

Gene gun

C

'Disarmed pathogen' vectors

D

Bacteriophages

Question 221: Palette 221/339

Allen MCQ

Refer to the following statements for agarose-gel electrophoresis:-
(a) Agarose is a natural polymer obtained from seaweed.
(b) The separation of DNA molecules in agarose-gel electrophoresis depends on the size of DNA.
(c) The DNA migrates from negatively-charged electrode to the positively-charged electrode.
(d) The DNA migrates from positively-charged electrode to the negatively-charged electrode.
Choose the most appropriate answer from the options given below:-

A

(a) and (b) only

B

(a), (b) and (c) only

C

(a), (c) and (d) only

D

(b), (c) and (d) only

Question 222: Palette 222/339

Allen MCQ

Milk of transgenic 'Cow Rosie' was nutritionally more balanced product for human babies than natural cow milk because it contained:-

A

Human protein $\alpha$-1-antitrypsin

B

Human alpha-lactalbumin

C

Human insulin-like growth factor

D

Human enzyme Adenosine Deaminase (ADA)

Question 223: Palette 223/339

Allen MCQ

The science, which deals with techniques of using live organisms or enzymes from organism to produce products and processes useful to human is :-

A

Genetics

B

Biotechnology

C

Bioinformatics

D

None of these

Question 224: Palette 224/339

Allen MCQ

A restriction endonucleases which always cut DNA molecules at a particular point by recognising a specific sequence of six base pairs is :-

A

Hind II

B

Pst I

C

Hae III

D

All of these

Question 225: Palette 225/339

Allen MCQ

The first letter of the name of Restriction endonuclease came from the:-

A

Genus of organism

B

Species of organism

C

Family of organism

D

Class of organism

Question 226: Palette 226/339

Allen MCQ

Autonomously replicating circular extra chromosomal DNA of bacteria is :-

A

Plastid

B

Nucleus

C

Plasmid

D

None of these

Question 227: Palette 227/339

Allen MCQ

The specific DNA sequence in a chromosome which is responsible for initiation of replication is :-

A

Cloning region

B

Termination region

C

Initiation region

D

Origin of replication

Question 228: Palette 228/339

Allen MCQ

Which of the following enzymes is known as 'genetic glue'?

A

DNA polymerase

B

Alkaline phosphatase

C

DNA ligase

D

All of the above

Question 229: Palette 229/339

Allen MCQ

pBR322 has two antibiotic resistance genes, they are :-

A

Streptomycin and Ampicillin resistant gene

B

Chloromycetin and tetracycline resistant gene

C

Tetracycline and neomycin resistant genes

D

Ampicillin and tetracyclin resistant genes

Question 230: Palette 230/339

Allen MCQ

To isolate DNA from the plant cells we have to break the wall this is done by :-

A

Lysozyme

B

Cellulase

C

Chitinase

D

Invertase

Question 231: Palette 231/339

Allen MCQ

Restriction enzymes belong to a larger class of enzymes called :-

A

Cellulases

B

Hydrolases

C

Polymerases

D

Nucleases

Question 232: Palette 232/339

Allen MCQ

The construction of the first recombinant DNA was done by ?

A

Stanley cohen and Herbert Boyer

B

Nathan's and Smith

C

Maeselson and Stahl

D

Allec Jeffreys

Question 233: Palette 233/339

Allen MCQ

EcoR I recognises palindromic sequence:-

A

5'GGGCCC3' 3'CCCGGG

B

5'-GAATTC-3' 3-CTTAAG-5'

C

5-AAGCTT3' 3-TTCGAA-5

D

None of the above

Question 234: Palette 234/339

Allen MCQ

Vector which is commonly used to transfer foreign gene in a crop plant is :-

A

Plasmids of Salmonella

B

$\lambda\text{ bacteriophage vector}$

C

Ti plasmid of Agrobacterium tumifaciens

D

None of the above

Question 235: Palette 235/339

Allen MCQ

Father of genetic engineering is :-

A

Paul Berg

B

Nathans

C

Herbert Boyer

D

Stanley Cohen

Question 236: Palette 236/339

Allen MCQ

A definition of biotechnology that encompasses both traditional view and modern view are given by :-

A

European forum on Biotechnology

B

European focus on Biotechnology

C

European Federation of Biotechnology

D

European Centre of Biotechnology

Question 237: Palette 237/339

Allen MCQ

Which one of the following is must in Genetic engineering?

A

Restriction endonuclease + DNA ligase

B

Restriction exonuclease + DNA polymerase

C

Alkaline phosphate + DNA Ligase

D

RNA polymerase + DNA polymerase

Question 238: Palette 238/339

Allen MCQ

Roman numbers following the names of restriction endonuclease indicate :-

A

The order in which the enzymes were isolated from that strain of bacteria

B

strain of bacteria

C

the order in which genus is taken to isolate the enzyme

D

none of the above

Question 239: Palette 239/339

Allen MCQ

Exonuclease removes nucleotides from:-

A

Specific positions

B

the ends of the DNA

C

any where in DNA

D

All the above

Question 240: Palette 240/339

Allen MCQ

In a chromosome there is a specific DNA sequence which is responsible for initiating replication is :-

A

Ori

B

Palindromic sequence

C

Initiation sequence

D

Promoter sequence

Question 241: Palette 241/339

Allen MCQ

The first restriction endonuclease discovered was:-

A

EcoR I

B

Sam I

C

BamH I

D

Hind II

Question 242: Palette 242/339

Allen MCQ

In the vector pBR322 there is :-

A

One selectable marker

B

Two selectable markers

C

Three selectable markers

D

None of the above

Question 243: Palette 243/339

Allen MCQ

When the isolation of genetic material is done the RNA can be removed by treatment with :-

A

Protease

B

Chitinase

C

Ribonuclease

D

Deoxyribonuclease

Question 244: Palette 244/339

Allen MCQ

Knife of DNA :-

A

DNA - ligase

B

Restriction endonuclease

C

Exonuclease

D

Peptidase

Question 245: Palette 245/339

Allen MCQ

The enzymes, which remove nucleotides from the ends of the DNA are :-

A

Exonuclease

B

Endonuclease

C

Cellulase

D

Hydrolase

Question 246: Palette 246/339

Allen MCQ

Group of letters that form the same words when read both forward and backward is called :-

A

Palindrome

B

Same words

C

Opposite words

D

None of the above

Question 247: Palette 247/339

Allen MCQ

Which type of ends are produced by EcoR I?

A

Blunt ends

B

Sticky ends

C

Both (1) and (2)

D

None of the above

Question 248: Palette 248/339

Allen MCQ

The sequence which is responsible for controlling the copy number of the linked DNA is :-

A

Coding sequence

B

Promoter sequence

C

Terminator sequence

D

Ori

Question 249: Palette 249/339

Allen MCQ

Apart from DNA in the bacterial nucleoid, there is a circular extrachromosomal DNA in a bacterial cell called :-

A

Plasmid

B

Mesosomes

C

Chromosome

D

None of these

Question 250: Palette 250/339

Allen MCQ

The stickiness of the ends, facilitates the action of enzyme :-

A

DNA ligase

B

DNA polymerase

C

Alkaline phosphatase

D

All of the above

Question 251: Palette 251/339

Allen MCQ

The linking of antibiotic resistant gene in the plasmid vector become possible with the enzyme :-

A

Restriction endonuclease

B

DNA ligase

C

DNA polymerase

D

RNA polymerase

Question 252: Palette 252/339

Allen MCQ

If any protein encoding gene is expressed in a heterologous host then protein is known as:-

A

Recombinant gene

B

Recombinant protein

C

Selectable marker

D

Homogenous protein

Question 253: Palette 253/339

Allen MCQ

The normal E. coli cell carries resistance gene against:-

A

Ampicillin

B

Chloramphenicol

C

Tetracycline

D

None of the above

Question 254: Palette 254/339

Allen MCQ

Taq polymerase is used in, polymerase chain reaction, because:-

A

It becomes inactive at high temperature

B

It makes other enzyme active at high temperature

C

It remains active during high temperature

D

It is obtained from thermostable virus

Question 255: Palette 255/339

Allen MCQ

The vessels, where large volumes of culture can be processed are:-

A

Bioreactors

B

Biovessels

C

Biocontainers

D

All of above

Question 256: Palette 256/339

Allen MCQ

Small chemically synthesised oligonucleotides that are complementary to the regions of DNA at 3' end used in "PCR" are:-

A

Primers

B

Dimers

C

Small strands

D

Large fragments

Question 257: Palette 257/339

Allen MCQ

Bombardment of high velocity micro-particles of gold or tungsten coated with DNA on target cells is:-

A

BioColumnics

B

Micro-injection

C

Electroporation

D

Bombing

Question 258: Palette 258/339

Allen MCQ

In micro injection:-

A

DNA is bombarded on target cells

B

DNA is placed through a vector

C

DNA is directly injected into the nucleus of animal cell

D

None of the above

Question 259: Palette 259/339

Allen MCQ

Most common matrix is agarose a natural polymer used in gel electrophoresis is extracted from:-

A

an animal

B

a fungus

C

sea weeds

D

None of these

Question 260: Palette 260/339

Allen MCQ

Agrobacterium tumifaciens a pathogen transform normal plant cells into a tumor, similarly in animals the normal cells transformed into cancerous cells by:-

A

Retro viruses

B

DNA viruses

C

Ribo viruses

D

None of these

Question 261: Palette 261/339

Allen MCQ

Insertional inactivation results into inactivation of which enzyme?

A

Transacetylase

B

Permease

C

Taq polymerase

D

$\beta$-galactosidase

Question 262: Palette 262/339

Allen MCQ

If the bacterium does not have any insert, then the presence of chromogenic substrate, it gives:-

A

Red coloured colonies

B

Colourless colonies

C

Blue colonies

D

Green colonies

Question 263: Palette 263/339

Allen MCQ

To make cell competent to take up DNA, heat shock is given to cells, the temperature of shock is:-

A

30°C

B

42°C

C

60°C

D

90°C

Question 264: Palette 264/339

Allen MCQ

In gel electrophoresis technique the DNA fragments are forced to move through a medium towards:-

A

Anode

B

Cathode

C

Both (1) and (2)

D

None of the above

Question 265: Palette 265/339

Allen MCQ

Which one is not a basic step in genetically modifying an organism?

A

Identification of DNA with desirable genes

B

Introduction of the identified DNA into the host

C

Introduction of unidentified DNA into the host

D

Maintenance of introduced DNA in the host and transfer of the DNA to its progeny

Question 266: Palette 266/339

Allen MCQ

The most commonly used bioreactors are of:-

A

Simple stirring type

B

Sparged stirring type

C

Both 1 and 2

D

None of the above

Question 267: Palette 267/339

Allen MCQ

Downstream processing is:-

A

Process of separation of DNA fragments

B

Process of joining the vector and the host DNA

C

Process including separation and purification of the product

D

Process of transferring DNA.

Question 268: Palette 268/339

Allen MCQ

Taq. polymerase is obtained from :-

A

Bacillus thuriengiensis

B

Thermus aquaticus

C

Salmonella typhimurium

D

Eischerichia coli

Question 269: Palette 269/339

Allen MCQ

To denature the DNA template in "PCR" it is heated to

A

70°C

B

54°C

C

80°C

D

94°C

Question 270: Palette 270/339

Allen MCQ

Alternative selectable markers developed to differentiate non-recombinants from recombinants on the basis of :-

A

Ability of separate them according to size

B

Ability to produce colour in the presence of a chromogenic substrate

C

Ability to not produce colour

D

None of the above

Question 271: Palette 271/339

Allen MCQ

If DNA is inserted within the coding sequence of $\beta$ -galactosidase enzyme then

A

Non-recombinants will give blue coloured colonies in presence of chromogenic substrate

B

Recombinant will give blue coloured colonies in presence of chromogenic substrate

C

Both recombinants and nonrecombinants give blue colour

D

Non-recombinants do not produce colour due to insertional inactivation.

Question 272: Palette 272/339

Allen MCQ

In gel electrophoresis the DNA fragments separate according to size (smaller the fragment size, the faster it moves) this effect is called :-

A

Sieving effect

B

Movement effect

C

Size effect

D

Spooling

Question 273: Palette 273/339

Allen MCQ

Extraction, purification and packaging of products is collectively known as :-

A

Upstream processing

B

Distillation

C

Downstream processing

D

Genetic engineering

Question 274: Palette 274/339

Allen MCQ

You have three copies of a particular DNA molecule what technique would you use to make more copies of the molecule?

A

Gel electrophoresis

B

Sequencing

C

"PCR"

D

Restriction fragment analysis

Question 275: Palette 275/339

Allen MCQ

Ti plasmid is present in :-

A

E.coli

B

Agrobacterium tumefaciens

C

Agrobacterium orifaciens

D

Vibrio cholera

Question 276: Palette 276/339

Allen MCQ

DNA cannot pass through cell membrane as it is

A

hydrophilic

B

hydrophobic

C

lipophilic

D

All the above

Question 277: Palette 277/339

Allen MCQ

Which type of bioreactor is usually cylindrical or with a curved base to facilitate the mixing of the contents?

A

Sparged tank bioreactor

B

Stirred tank bioreactor

C

Both (1) and (2)

D

None of the above

Question 278: Palette 278/339

Allen MCQ

In "PCR"-technology primer is a :-

A

Small chemically synthesized oligonucleotide that are complementary to region of DNA

B

Large chemically synthesized oligonucleotide that are identical to region of DNA

C

Small segment of RNA

D

None of these

Question 279: Palette 279/339

Allen MCQ

In gel electrophoresis the DNA fragment separate according to their size through sieving effect, which is provided by :-

A

Agarose gel

B

Nylone membrane

C

Polyethylene glycol

D

Ethidium Bromide

Question 280: Palette 280/339

Allen MCQ

Which of the following method of vectorless gene transfer is suitable for plants ?

A

BioColumnics method

B

Micro injection

C

Liposome mediated

D

Electroporation

Question 281: Palette 281/339

Allen MCQ

In gel electrophoresis, separated bands of DNA are cut out from the agarose gel and extracted from the gel pieces, This step is known as :-

A

Blotting

B

Elution

C

Cloning

D

Taggings

Question 282: Palette 282/339

Allen MCQ

Which enzyme is used in "PCR" technique ?

A

Thermostable DNA polymerase

B

Thermostable RNA polymerase

C

Thermostable ligase

D

Thermostable vector

Question 283: Palette 283/339

Allen MCQ

Which of the following is used to deliver desirable gene in to animal cell?

A

Disarmed retrovirus

B

Disarmed agrobacterium

C

Disarmed E. coli

D

Disarmed plant pathogen

Question 284: Palette 284/339

Allen MCQ

Agrobacterium tumifaciencs, a pathogen of several dicot plants is able to deliver a piece of DNA and it is known as :-

A

R-DNA

B

S-DNA

C

M-DNA

D

T-DNA

Question 285: Palette 285/339

Allen MCQ

A transgenic food crop, which may help in solving the problem of night blindness in developing countries is :-

A

Bt soyabean

B

Golden rice

C

Flavr savr tomatoes

D

Starlink maize

Question 286: Palette 286/339

Allen MCQ

Bacterium Bacillus thuringiensis is widely used in contemporary biology as

A

Source of industrial enzyme

B

Indicator of water pollution

C

Insecticide

D

Agent for production of dairy products.

Question 287: Palette 287/339

Allen MCQ

Bacillus thuringiensis is a :-

A

Bacterium

B

Protozoa

C

Fungus

D

Virus

Question 288: Palette 288/339

Allen MCQ

GEAC stands for :-

A

Gene evaluation approval committee

B

Genetic engineering approval committee

C

Genetic engineering applied committee

D

Gene enhancement approval committee

Question 289: Palette 289/339

Allen MCQ

Conventional methods to diagnose a disease are :-

A

Serum and urine analysis

B

""

C

ELISA

D

All of the above

Question 290: Palette 290/339

Allen MCQ

The first transgenic cow, which produced human protein enriched milk was named :-

A

Andy

B

Dolly

C

Rosie

D

Dumpy

Question 291: Palette 291/339

Allen MCQ

Milk of transgenic cow 'Rosie' contains a substance that was nutritionally more balanced product for human babies is :-

A

$\alpha\text{-lactalbumin}$

B

$\beta\text{-lactalbumin}$

C

$\gamma\text{-lactalbumin}$

D

$\delta\text{-lactalbumin}$

Question 292: Palette 292/339

Allen MCQ

Which peptide is not present in the mature insulin and is removed during maturation into insulin?

A

A-peptide

B

B-peptide

C

C-peptide

D

Both (1) and (2)

Question 293: Palette 293/339

Allen MCQ

Two polypeptide chains of insulin are linked together by :-

A

disulphide bonds

B

hydrogen bonds

C

Phosphodiester bonds

D

Glycosidic bonds

Question 294: Palette 294/339

Allen MCQ

'Flavr Savr' is a transgenic variety of :-

A

Potato

B

Tomato

C

Soyabean

D

Rice

Question 295: Palette 295/339

Allen MCQ

Golden rice is enriched in :-

A

Vitamin C

B

Vitamin D

C

Vitamin A

D

Vitamin E

Question 296: Palette 296/339

Allen MCQ

For the control of the cotton bollworms, which one of the genes is useful ?

A

cry I Ac

B

cry I Ab

C

cry I Ad

D

All of these

Question 297: Palette 297/339

Allen MCQ

Process involving silencing of a specific mRNA due to a complementary dsRNA molecule is called :-

A

Transcription

B

RNA interference

C

DNA interference

D

None of these

Question 298: Palette 298/339

Allen MCQ

Meloidegyne incognitia which infects the roots of tobacco plants causing a great reduction in yield is a :-

A

Nematode

B

Bacterium

C

Virus

D

Alga

Question 299: Palette 299/339

Allen MCQ

Toxin present in Bacillus thuringiensis does not kill the bacterium because it is inactive form what makes it active inside the insect?

A

the alkaline pH of the gut, which solubilises the crystals

B

the acid pH of the gut

C

the neutral pH of the gut

D

All of the above

Question 300: Palette 300/339

Allen MCQ

Crystals of Bt toxin produced by some bacteria do not kill the bacteria themselves because :-

A

bacteria are resistant to the toxin

B

toxin is immature

C

toxin is inactive

D

bacteria enclose toxin in a special sac

Question 301: Palette 301/339

Allen MCQ

Which one of the following statements about genetically engineered insulin is incorrect?

A

E. coli is used for producing human insulin?

B

Chains A, B were produced separately

C

Eli lilly company prepared it for first time

D

Genetically engineered insulin has C-peptide

Question 302: Palette 302/339

Allen MCQ

Infection by pathogen can be detected by the presence of antigens or by detecting the antibodies synthesised against the pathogen, on this principle a test is based which is?

A

"PCR"

B

ELISA

C

Both 1 and 2

D

None of the above

Question 303: Palette 303/339

Allen MCQ

Indian parliament recently cleared, which amendment of the Indian patents bill:-

A

First amendment

B

Second amendment

C

Third amendment

D

Fourth amendment

Question 304: Palette 304/339

Allen MCQ

How many documented varieties of basmati rice distinct for its unique aroma and flavour are grown in India?

A

27 varieties

B

25 varieties

C

28 varieties

D

26 varieties

Question 305: Palette 305/339

Allen MCQ

Over 95 percent of all existing transgenic animals are:-

A

Pigs

B

Cows

C

Fish

D

Mice

Question 306: Palette 306/339

Allen MCQ

The organisation set up for making decisions regarding the validity of GM research and the safety of introducing GM organism for public services is:-

A

Genetic engineering approval committee

B

Genetic engineering advanced company

C

Genetic engineering applied committee

D

None of these

Question 307: Palette 307/339

Allen MCQ

Use of bio-resources by multinational companies and other organisations without proper authorisation from the countries and people concerned without compensatory payment is called:-

A

Bio theft

B

Biopatent

C

Biopiracy

D

None of the above

Question 308: Palette 308/339

Allen MCQ

Which animal is being used to test the safety of polio vaccine?

A

Transgenic mice

B

Transgenic pig

C

Transgenic cow

D

Transgenic cat

Question 309: Palette 309/339

Allen MCQ

Cry1Ab gene produces proteins which control?

A

Bollworms

B

Corn borer

C

Both A and B

D

None of the above

Question 310: Palette 310/339

Allen MCQ

Nematode-specific genes were introduced into the host plant (tobacco plant) by using which vector?

A

Plasmid vector

B

Cosmid vector

C

Bacteriophage vector

D

BAC

Question 311: Palette 311/339

Allen MCQ

Which one of the following statements are true regarding genetic modifications?

A

Genetic modifications reduced reliance on chemical pesticides

B

Genetic modifications has enhanced nutritional value of food.

C

Genetic modifications made crops more tolerant to abiotic stresses.

D

All are correct

Question 312: Palette 312/339

Allen MCQ

Critical research areas of biotechnology are:-

A

providing the best catalyst in the form of improved organism usually a microbe or pure enzyme.

B

Creating optimal conditions through engineering for a catalyst to act.

C

Down stream processing technologies to purify the protein/organic compound.

D

All the above

Question 313: Palette 313/339

Allen MCQ

Bacterium genetically engineered for cleaning oil spills is:-

A

Escherichia coli

B

Pseudomonas putida

C

Salmonella typhimurium

D

Agrobacterium tumifaciens

Question 314: Palette 314/339

Allen MCQ

Bacterium which is known as 'Super bug' is:-

A

Pseudomonas putida

B

Salmonella

C

Escherichia

D

Agrobacterium

Question 315: Palette 315/339

Allen MCQ

Animals those have had their DNA manipulated to possess and express an extra (foreign) gene are known as:-

A

Transgenic animals

B

Genetically modified animals

C

Both 1 and 2

D

None of the above

Question 316: Palette 316/339

Allen MCQ

When cut by the restriction enzyme, the DNA fragments can be joined together using :-

A

DNA polymerase

B

DNA ligase

C

Alkaline phosphatase

D

DNA gyrase

Question 317: Palette 317/339

Allen MCQ

Genetically engineered human insulin is made in:-

A

Fungus

B

Protista

C

Plants

D

Bacterium

Question 318: Palette 318/339

Allen MCQ

Genetically engineered bacteria are being used in commercial production of :-

A

melatonin

B

testosterone

C

thyroxine

D

human insulin

Question 319: Palette 319/339

Allen MCQ

Insulin consists of two short polypeptide chains, which are linked together by?

A

Sulphide bridges

B

Peptide bridges

C

Chloride bridges

D

Disulphide bridges

Question 320: Palette 320/339

Allen MCQ

Meloidegyne incognitia infects the root of which plant ?

A

Potato

B

Soyabean

C

Tobacco

D

Tomato

Question 321: Palette 321/339

Allen MCQ

Genetics modified crops (GMC) are useful in agriculture because :-

A

They are more tolerant to abiotic stresses

B

They increase reliance on chemical pesticide

C

They have reduced nutritional value

D

All the above

Question 322: Palette 322/339

Allen MCQ

A nematode Meloidegyne incognitia infects the root of tobacco plant and causes a great reduction in yield. A novel strategy was adopted to prevent this infection which was based on the process of :-

A

DNA interference

B

RNA interference

C

"" technique

D

DNA test

Question 323: Palette 323/339

Allen MCQ

In RNA interference (RNAi) :-

A

The silencing of a specific mRNA due to a complementary dsRNA molecule that binds to and prevent translation of the mRNA

B

The silencing of a specific mRNA due to dsDNA

C

The silencing of a specific mRNA due to tRNA

D

All the above

Question 324: Palette 324/339

Allen MCQ

The protein encoded by the gene cryIAc and cryIIAb, controls :-

A

Cotton bollworm

B

Corn borer

C

Cotton borer

D

All the above

Question 325: Palette 325/339

Allen MCQ

Transgenic animals produces biological product such as $\alpha$-1-antitrypsin, which is used to treat :-

A

Emphysema

B

Cystic fibrosis

C

Phenyl ketonuria

D

Sickle cell anaemia

Question 326: Palette 326/339

Allen MCQ

The first transgenic cow was 'Rosie', produces :-

A

Human protein-enriched milk ($\alpha$-lactabumin)

B

Human protein $\alpha$-1 antitrypsin riched milk

C

Human protein enriched milk (insulin)

D

All the above

Question 327: Palette 327/339

Allen MCQ

Transgenic mice are being developed for use in

A

Testing the safety of polio vaccines before they are used on human

B

Molecular diagnosis of diseases

C

Production of human protein enriched milk

D

Production of human insulin

Question 328: Palette 328/339

Allen MCQ

The technique that serves the purpose of early diagnosis of disease or pathogen :-

A

Recombinant DNA technology

B

Polymerase chain reaction ("")

C

Enzyme linked immuno sorbent assay (ELISA)

D

All the above

Question 329: Palette 329/339

Allen MCQ

Transgenic tobacco which is developed through RNA interference, prevents the infection of :-

A

A nematode - Meloidegyne incognitia

B

A bacterium - Pseudonomonas putida

C

A fungi - Tricoderma

D

An insect

Question 330: Palette 330/339

Allen MCQ

The basic procedure involved in the synthesis of recombinant DNA molecule is depicted below. The mistake in the procedure is :-

A

Enzyme polymerase is not included

B

The mammalian DNA is shown double stranded

C

Only one fragment is inserted

D

Two different restriction enzymes are used

Question 331: Palette 331/339

Allen MCQ

Production of a human protein in bacteria by genetic engineering is possible because

A

Bacterial cell can carry out the RNA splicing reactions

B

The mechanism of gene regulation is identical in humans and bacteria

C

The human chromosome can replicate in bacterial cell

D

The genetic code is universal

Question 332: Palette 332/339

Allen MCQ

If a recombinant DNA bearing gene for ampicillin resistance if transferred into E. coli cells and the host cells are spread on agar plates containing ampicillin, then :-

A

both transformed and untransformed recipient cells will die

B

both transformed and untransformed recipient cell will be grow

C

transformed recipient cells will grow and untransformed recipient cells will die

D

transformed recipient cells will die and untransformed recipient cells will grow

Question 333: Palette 333/339

Allen MCQ

If hemoglobin (Hb) of a normal individual and a sickle-cell patient are run in electrophoretic field, they will show :-

A

same mobilities

B

different mobilities

C

Hb of patient will not move at all

D

Hbs are immobile

Question 334: Palette 334/339

Allen MCQ

Introduction of food plants developed by genetic engineering is not desirable because–

A

Economy of developing countries may suffer.

B

These products are less tasty as compared to the already existing products.

C

This method is costly.

D

There is danger of coming viruses and toxins with introduced crop.

Question 335: Palette 335/339

Allen MCQ

Which one of the following is a correct statement ?

A

"Bt" in "Bt-cotton" indicates that it is a genetically modified organism produced through biotechnology

B

Somatic hybridization involves fusion of two complete plant cells carrying desired genes.

C

The anticoagulant hirudin is being produced from transgenic Brassica napus seeds.

D

"Flavr Savr" variety of tomato has enhanced the production of ethylene which improves its taste.

Question 336: Palette 336/339

Allen MCQ

Which of the following tools of recombinant DNA technology is incorrectly paired with its use ?

A

restriction enzyme - Production of RFLPs

B

DNA ligase-that cuts DNA, creating the sticky ends

C

DNA polymerase - used in a polymerase chain reaction to amplify section of DNA

D

reverse transcriptase - production of cDNA from mRNA

Question 337: Palette 337/339

Allen MCQ

Select the incorrect statement for continuous culture system:–

A

In this used medium is drained out from one side while fresh medium is added from other side.

B

In this cells are maintained in their physiologically most active lag phase of growth.

C

It produces larger biomass.

D

It shows higher yields of desired product.

Question 338: Palette 338/339

Allen MCQ

In r-DNA technology or genetic engineering elution means :-

A

Remove the DNA from centrifuge tube after centrifugation

B

The separated band of DNA are cut out from the gel and extracted from the gel piece

C

Separation of the recombinant protein from recombinant cell

D

Insertion of recombinant DNA into the host cell

Question 339: Palette 339/339

Allen MCQ

An example of gene therapy is:-

A

Production of injectable Hepatitis-B vaccine

B

Production of vaccines in food crops like potatoes which can be eaten

C

Introduction of gene for adenosine deaminase in persons suffering from severe combined immuno-deficiency (SCID)

D

Production of test tube babies by artificial insemination and implantation of fertilized eggs