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Biotechnology And Its Applications

339 Questions Start Allen Test
All Exams Allen
Q201 Allen 3. PYQ MCQ
Which of the following is a correct sequence of steps in a "PCR" (Polymerase Chain Reaction) ?
A.
Denaturation, Annealing, Extension
B.
Denaturation, Extension, Annealing
C.
Extension, Denaturation, Annealing
D.
Annealing, Denaturation, Extension
Q202 Allen 3. PYQ MCQ
DNA strands on a gel stained with ethidium bromide when viewed under UV radiation, appear as :-
A.
Yellow bands
B.
Bright orange bands
C.
Dark red bands
D.
Bright blue bands
Q203 Allen 3. PYQ MCQ
When gene targetting involving gene amplification is attempted in an individual's tissue to treat disease, it is known as :-
A.
Biopiracy
B.
Gene therapy
C.
Molecular diagnosis
D.
Safety testing
Q204 Allen 3. PYQ MCQ
Which of the following is not an application of "PCR" (Polymerase Chain Reaction) ?
A.
Molecular diagnosis
B.
Gene amplification
C.
Purification of isolated protein
D.
Detection of gene mutation
Q205 Allen 3. PYQ MCQ
Plasmid pBR322 has PstI restriction enzyme site within gene ampR that confers ampicillin resistance. If this enzyme is used for inserting a gene for Ī²-galactoside production and the recombinant plasmid is inserted in an E.coli strain
A.
it will not be able to confer ampicillin resistance to the host cell.
B.
the transformed cells will have the ability to resist ampicillin as well as produce Ī²- galactoside.
C.
it will lead to lysis of host cell.
D.
it will be able to produce a novel protein with dual ability.
Q206 Allen 3. PYQ MCQ
A specific recognition sequence identified by endonucleases to make cuts at specific positions within the DNA is :-
A.
Degenerate primer sequence
B.
Okazaki sequences
C.
Palindromic Nucleotide sequences
D.
Poly(A) tail sequences
Q207 Allen 3. PYQ MCQ
With regard to insulin choose correct options.
(a) C-peptide is not present in mature insulin.
(b) The insulin produced by rDNA technology has C-peptide.
(c) The pro-insulin has C-peptide.
(d) A-peptide and B-peptide of insulin are interconnected by disulphide bridges.
Choose the correct answer from the options given below.
A.
(b) and (d) only
B.
(b) and (c) only
C.
(a), (c) and (d) only
D.
(a) and (d) only
Q208 Allen 3. PYQ MCQ
During the process of gene amplification using "PCR", if very high temperature is not maintained in the beginning, then which of the following steps of "PCR" will be affected first?
A.
Annealing
B.
Extension
C.
Denaturation
D.
Ligation
Q209 Allen 3. PYQ MCQ
For effective treatment of the disease, early diagnosis and understanding its pathophysiology is very important. Which of the following molecular diagnostic techniques is very useful for early detection?
A.
Western Blotting Technique
B.
Southern Blotting Technique
C.
ELISA Technique
D.
Hybridization Technique
Q210 Allen 3. PYQ MCQ
Which one of the following statement is not true regarding gel electrophoresis technique?
A.
The separated DNA fragments are stained by using ethidium bromide.
B.
The presence of chromogenic substrate gives blue coloured DNA bands on the gel.
C.
Bright orange coloured bands of DNA can be observed in the gel when exposed to UV light.
D.
The process of extraction of separated DNA strands from gel is called elution.
Q211 Allen 3. PYQ MCQ
Given below are two statements :-
one is labelled as Assertion (A) and the other is labelled as Reason (R).
Assertion (A) : Polymerase chain reaction is used in DNA amplification
Reason (R) : The ampicillin resistant gene is used as a selectable marker to check transformation.
In the light of the above statements,
choose the correct answer from the options given below:-
A.
Both (A) and (R) are correct but (R) is not the correct explanation of (A)
B.
(A) is correct but (R) is not correct
C.
(A) is not correct but (R) is correct
D.
Both (A) and (R) are correct and (R) is the correct explanation of (A)
Q212 Allen 3. PYQ MCQ
In the following palindromic base sequences of DNA, which one can be cut easily by particular restriction enzyme ?
A.
5' G A A T T C 3'; 3' C T T A A G 5'
B.
5' C T C A G T 3'; 3' G A G T C A 5'
C.
5' G T A T T C 3'; 3' C A T A A G 5'
D.
5' G A T A C T 3'; 3' C T A T G A 5'
Q213 Allen 3. PYQ MCQ
Given below are two statements:- Statement I : Restriction endonucleases recognise specific sequence to cut DNA known as palindromic nucleotide sequence. Statement II : Restriction endonucleases cut the DNA strand a little away from the centre of the palindromic site. In the light of the above statements, choose the most appropriate answer from the options given below:-
A.
Both Statement I and Statement II are incorrect
B.
Statement I is correct but Statement II is incorrect
C.
Statement I is incorrect but Statement II is correct
D.
Both Statement I and Statement II are correct
Q214 Allen 3. PYQ MCQ
Which of the following is not a desirable feature of a cloning vector ?
A.
Presence of a marker gene
B.
Presence of single restriction enzyme site
C.
Presence of two or more recognition sites
D.
Presence of origin of replication
Q215 Allen 3. PYQ MCQ
Transposons can be used during which one of the following?
A.
Gene silencing
B.
Autoradiography
C.
Gene sequencing
D.
Polymerase Chain Reaction
Q216 Allen 3. PYQ MCQ
Statements related to human Insulin are given below :-
Which statement(s) is/ are correct about genetically engineered Insulin ?
(a) Pro-hormone insulin contain extra stretch of C-peptide
(b) A-peptide and B-peptide chains of insulin were produced separately in E.coli, extracted and combined by creating disulphide bond between them.
(c) Insulin used for treating Diabetes was extracted from Cattles and Pigs.
(d) Pro-hormone Insulin needs to be processed for converting into a mature and functional hormone.
(e) Some patients develop allergic reactions to the foreign insulin.
Choose the most appropriate answer from the options given below:-
A.
(b)only
B.
(c) and (d) only
C.
(c), (d) and (e) only
D.
(a), (b) and (d) only
Q217 Allen 3. PYQ MCQ
Separation of DNA, fragments is done by a technique known as :-
A.
Polymerase Chain Reaction
B.
Recombinant technology
C.
Southern blotting
D.
Gel electrophoresis
Q218 Allen 3. PYQ Match the Columns
Match Column-I with Column-II :- Column-I Column-II
Column-IColumn-II
(A)Gene gun
(B)Gene therapy
(C)Gene cloning
(D)Genome
(I)Replacement of a faulty gene by a normal healthy gene
(II)Used for transfer of gene
(III)Total DNA in the cells of an organism
(IV)To obtain identical copies of a particular DNA molecule
A.
A-II, B-I, C-IV, D-III
B.
A-I, B-III, C-II, D-IV
C.
A-IV, B-I, C-III, D-II
D.
A-II, B-III, C-IV, D-I
Q219 Allen 3. PYQ MCQ
Pathogenic bacteria gain resistance to antibiotics due to changes in their :-
A.
Cosmids
B.
Plasmids
C.
Nucleus
D.
Nucleoid
Q220 Allen 3. PYQ MCQ
Which of the following methods is not commonly used for introducing foreign DNA into the plant cell?
A.
Agrobacterium mediated transformation
B.
Gene gun
C.
'Disarmed pathogen' vectors
D.
Bacteriophages
Q221 Allen 3. PYQ MCQ
Refer to the following statements for agarose-gel electrophoresis:-
(a) Agarose is a natural polymer obtained from seaweed.
(b) The separation of DNA molecules in agarose-gel electrophoresis depends on the size of DNA.
(c) The DNA migrates from negatively-charged electrode to the positively-charged electrode.
(d) The DNA migrates from positively-charged electrode to the negatively-charged electrode.
Choose the most appropriate answer from the options given below:-
A.
(a) and (b) only
B.
(a), (b) and (c) only
C.
(a), (c) and (d) only
D.
(b), (c) and (d) only
Q222 Allen 3. PYQ MCQ
Milk of transgenic 'Cow Rosie' was nutritionally more balanced product for human babies than natural cow milk because it contained:-
A.
Human protein $\alpha$-1-antitrypsin
B.
Human alpha-lactalbumin
C.
Human insulin-like growth factor
D.
Human enzyme Adenosine Deaminase (ADA)
Q223 Allen 4. NCERT BASED QUESTIONS MCQ
The science, which deals with techniques of using live organisms or enzymes from organism to produce products and processes useful to human is :-
A.
Genetics
B.
Biotechnology
C.
Bioinformatics
D.
None of these
Q224 Allen 4. NCERT BASED QUESTIONS MCQ
A restriction endonucleases which always cut DNA molecules at a particular point by recognising a specific sequence of six base pairs is :-
A.
Hind II
B.
Pst I
C.
Hae III
D.
All of these
Q225 Allen 4. NCERT BASED QUESTIONS MCQ
The first letter of the name of Restriction endonuclease came from the:-
A.
Genus of organism
B.
Species of organism
C.
Family of organism
D.
Class of organism