← Back to Zoology

Biotechnology And Its Applications

339 Questions Start Allen Test
All Exams Allen
Q176 Allen 3. PYQ MCQ
What triggers activation of protoxin to active Bt toxin of Bacillus thuringiensis in boll worm?
A.
Body temperature
B.
Moist surface of midgut
C.
Alkaline pH of gut
D.
Acidic pH of stomach
Q177 Allen 3. PYQ Match the Columns
Match the following enzymes with their functions:-
Match Column-I with Column-II
Column-IColumn-II
(A)Restriction endonuclease
(B)Restriction exonuclease
(C)DNA ligase
(D)Taq polymerase
(I)Joins the DNA fragements
(II)Extends primers on genomic DNA template
(III)Cuts DNA at specific position
(IV)Removes nucleotides from the ends of DNA
A.
A-III, B-I, C-IV, D-II
B.
A-III, B-IV, C-I, D-II
C.
A-IV, B-III, C-I, D-II
D.
A-II, B-IV, C-I, D-III
Q178 Allen 3. PYQ MCQ
The two antibiotic resistance genes on vector pBR322 are :-
A.
Ampicillin and Tetracycline
B.
Ampicillin and Chloramphenicol
C.
Chloramphenicol and Tetracycline
D.
Tetracycline and Kanamycin
Q179 Allen 3. PYQ MCQ
Exploitation of bioresources of a nation by multinational companies without authorization from the concerned country is referred to as: -
A.
Bioweapon
B.
Biopiracy
C.
Bioethics
D.
Biowar
Q180 Allen 3. PYQ MCQ
A selectable marker is used to: -
A.
help in eliminating the non-transformants, so that the transformants can be regenerated
B.
identify the gene for a desired trait in an alien organism
C.
select a suitable vector for transformation in a specific crop
D.
mark a gene on a chromosome for isolation using restriction enzyme
Q181 Allen 3. PYQ MCQ
Given below are four statements pertaining to separation of DNA fragments using gel electrophoresis. Identify the incorrect statements: -
(a) DNA is negatively charged molecule and so it is loaded on gel towards the Anode terminal (b) DNA fragments travel along the surface of the gel whose concentration does not affect movement of DNA. (c) Smaller the size of DNA fragment larger is the distance it travels through it. (d) Pure DNA can be visualized directly by exposing UV radiation. Choose correct answer from the options given below
A.
(a), (c) and (d)
B.
(a), (b) and (c)
C.
(b), (c) and (d)
D.
(a), (b) and (d)
Q182 Allen 3. PYQ MCQ
An enzyme catalysing the removal of nucleotides from ends of DNA is:-
A.
DNA ligase
B.
Endonuclease
C.
Exonuclease
D.
Protease
Q183 Allen 3. PYQ MCQ
In RNAi, the genes are silenced using:-
A.
ds-RNA
B.
ss-DNA
C.
ss-RNA
D.
ds-DNA
Q184 Allen 3. PYQ MCQ
Which of the following statements is not correct?
A.
Genetically engineered insulin is produced in E coli.
B.
In man insulin is synthesised as a proinsulin.
C.
The proinsulin has an extra peptide called C-peptide.
D.
The functional insulin has A and B chains linked together by hydrogen bonds.
Q185 Allen 3. PYQ Match the Columns
Match the organism with its use in biotechnology.
Match Column-I with Column-II:
Column-IColumn-II
(A)Bacillus thuringiensis
(B)Thermus aquaticus
(C)Agrobacterium tumefaciens
(D)Salmonella typhimurium
(I)Cloning vector
(II)Construction of first rDNA molecule
(III)DNA polymerase
(IV)Cry proteins
A.
A-III, B-IV, C-I, D-II
B.
A-II, B-IV, C-III, D-I
C.
A-IV, B-III, C-I, D-II
D.
A-III, B-II, C-IV, D-I
Q186 Allen 3. PYQ MCQ
Bt cotton variety that was developed by the introduction of toxin gene of Bacillus thuringiensis (Bt) is resistant to :-
A.
Insect predators
B.
Insect pests
C.
Fungal diseases
D.
Plant nematodes
Q187 Allen 3. PYQ MCQ
The specific palindromic sequence which is recognized by EcoR I is :-
A.
5' - GGATCC - 3' , 3' - CCTAGG - 5'
B.
5' - GAATTC - 3' , 3' - CTTAAG - 5'
C.
5' - GGAACC - 3' , 3' - CCTTGG - 5'
D.
5' - CTTAAG - 3', 3' - GAATTC - 5'
Q188 Allen 3. PYQ MCQ
Choose the correct pair from the following:-
A.
Exonucleases: - Make cuts at specific positions within DNA
B.
Ligases :- Join the two DNA molecules
C.
Polymerases: Break the DNA into fragments
D.
Nucleases : Separate the two strands of DNA
Q189 Allen 3. PYQ Match the Columns
Match the following columns and select the correct
Match Column-I with Column-II:
Column-IColumn-II
(A)Bt cotton
(B)Adenosine deaminase
(C)RNAi
(D)"PCR"
(I)Gene therapy
(II)Cellular defence deficiency
(III)Detection of HIV infection
(IV)Bacillus thuringiensis
A.
A-I, B-II, C-III, D-IV
B.
A-IV, B-I, C-II, D-III
C.
A-III, B-II, C-I, D-IV
D.
A-II, B-III, C-IV, D-I
Q190 Allen 3. PYQ MCQ
Identify the wrong statement with regard to Restriction Enzymes.
A.
Sticky ends can be joined by using DNA ligases.
B.
Each restriction enzyme functions by inspecting the length of a DNA sequence.
C.
They cut the strand of DNA at palindromic sites.
D.
They are useful in genetic engineering.
Q191 Allen 3. PYQ MCQ
The sequence that controls the copy number of the linked DNA in the vector, is termed :-
A.
Recognition site
B.
Selectable marker
C.
Ori site
D.
Palindromic sequence
Q192 Allen 3. PYQ MCQ
First discovered restriction endonuclease that always cuts DNA molecule at a particular point by recognising a specific sequence of six base pairs is:-
A.
EcoR1
B.
Adenosine deaminase
C.
Thermostable DNA polymerase
D.
Hind II
Q193 Allen 3. PYQ MCQ
In Recombinant DNA technology antibiotics are used :-
A.
to keep medium bacteria-free
B.
to detect alien DNA
C.
to impart disease-resistance to the host plant
D.
as selectable markers
Q194 Allen 3. PYQ Match the Columns
Match the following techniques or instruments with their usage :- Match Column-I with Column-II:
Column-IColumn-II
(A)Bioreactor
(B)Electrophoresis
(C)"PCR"
(D)ELISA
(I)Separation of DNA fragments
(II)Production of large quantities of products
(III)Detection of pathogen, based on antigen ā€“ antibody reaction
(IV)Amplification of nucleic acids
A.
A-III, B-II, C-IV, D-I
B.
A-II, B-I, C-IV, D-III
C.
A-IV, B-III, C-II, D-I
D.
A-II, B-I, C-III, D-IV
Q195 Allen 3. PYQ MCQ
In a mixture, DNA fragments are separated by:-
A.
Bioprocess engineering
B.
Restriction digestion
C.
Electrophoresis
D.
Polymerase chain reaction
Q196 Allen 3. PYQ MCQ
Spooling is :-
B.
Cutting of separated DNA bands from the agarose gel
C.
Transfer of separated DNA fragments to synthetic membranes
D.
Collection of isolated DNA
Q197 Allen 3. PYQ MCQ
Select the correct statement from the following :-
A.
Gel electrophoresis is used for amplification of a DNA segment.
B.
The polymerase enzyme joins the gene of interest and the vector DNA.
C.
Restriction enzyme digestions are performed by incubating purified DNA molecules with the restriction enzymes of optimum conditions.
D.
"" is used for isolation and separation of gene of interest.
Q198 Allen 3. PYQ MCQ
RNA interference is used for which of the following purposes in the field of biotechnology ?
A.
to develop a plant tolerant to abiotic stresses
B.
to develop a pest resistant plant against infestation by nematode
C.
to enhance the mineral usage by the plant
D.
to reduce post-harvest losses
Q199 Allen 3. PYQ MCQ
The laws and rules to prevent unauthorised exploitation of bio-resources are termed as-
A.
Biopatenting
B.
Bioethics
C.
Bioengineering
D.
Biopiracy
Q200 Allen 3. PYQ MCQ
During the purification process for recombinant DNA technology, addition of chilled ethanol precipitates out:-
A.
RNA
B.
DNA
C.
Histones
D.
Polysaccharides