Biotechnology And Its Applications

339 Questions Start Allen Test
Q151 Allen 3. PYQ MCQ
Which body of the Government of India regulates GM research and safety of introducing GM organisms for public services?
A.
Indian Council of Agricultural Research
B.
Genetic Engineering Approval Committee
C.
Research Committee on Genetic Manipulation
D.
Bio-safety committee
Q152 Allen 3. PYQ MCQ
The DNA molecules to which the gene of interest is integrated for cloning is called :-
A.
Carrier
B.
Transformer
C.
Vector
D.
Template
Q153 Allen 3. PYQ MCQ
The cutting of DNA at specific locations became possible with the discovery of :-
A.
Ligases
B.
Restriction enzymes
C.
Probes
D.
Selectable markers
Q154 Allen 3. PYQ MCQ
Golden rice is a genetically modified crop plant where the incorporated gene is meant for biosynthesis of :-
A.
Vitamin A
B.
Vitamin B
C.
Vitamin C
D.
Omega 3
Q155 Allen 3. PYQ MCQ
The introduction of T-DNA into plants involves: -
A.
Allowing the plant roots to stand in water
B.
Infection of the plant by Agrobacterium tumefaciens
C.
Altering the pH of the soil, then heat shocking the plants
D.
Exposing the plants to cold for a brief period
Q156 Allen 3. PYQ MCQ
The two polypeptides of human insulin are linked together by :-
A.
Hydrogen bonds
B.
Phosphodiester bond
C.
Covalent bond
D.
Disulphide bridges
Q157 Allen 3. PYQ MCQ
Which of the following is not a feature of the plasmids ?
A.
Independent replication
B.
Circular structure
C.
Transferable
D.
Single - stranded
Q158 Allen 3. PYQ MCQ
The taq polymerase enzyme is obtained from:-
A.
Thermus aquaticus
B.
Thiobacillus ferroxidans
C.
Bacillus subtilis
D.
Pseudomonas putida
Q159 Allen 3. PYQ MCQ
Which of the following is a restriction endonuclease?
A.
Hind II
B.
Protease
C.
DNase I
D.
RNase
Q160 Allen 3. PYQ MCQ
Stirred-tank bioreactors have been designed for :-
A.
availability of oxygen throughout the process
B.
ensuring anaerobic conditions in the culture vessel
C.
purification of product
D.
addition of preservatives to the product
Q161 Allen 3. PYQ MCQ
A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using :-
A.
Polymerase-III
B.
Ligase
C.
Eco RI
D.
Taq polymerase
Q162 Allen 3. PYQ MCQ
Which of the following is not a component of downstream processing ?
A.
Preservation
B.
Expression
C.
Separation
D.
Purification
Q163 Allen 3. PYQ MCQ
Which of the following restriction enzymes produces blunt ends ?
A.
Xho I
B.
Hind III
C.
Sal I
D.
Eco RV
Q164 Allen 3. PYQ MCQ
The DNA fragments separated on an agarose gel can be visualised after staining with :-
A.
Acetocarmine
B.
Aniline blue
C.
Ethidium bromide
D.
Bromophenol blue
Q165 Allen 3. PYQ MCQ
The process of separation and purification of expressed protein before marketing is called:
A.
Downstream processing
B.
Bioprocessing
C.
Postproduction processing
D.
Upstream processing
Q166 Allen 3. PYQ MCQ
A gene whose expression helps to identify transformed cell is known as :-
A.
Vector
B.
Plasmid
C.
Structural gene
D.
Selectable marker
Q167 Allen 3. PYQ MCQ
Which of the following is commonly used as a vector for introducing a DNA fragment in human lymphocytes ?
A.
Retrovirus
B.
Ti plasmid
C.
$\lambda phage$
D.
pBR322
Q168 Allen 3. PYQ MCQ
In India, the organisation responsible for assessing the safety of introducing genetically modified organisms for public use is :-
A.
Indian Council of Medical Research (ICMR)
B.
Council for Scientific and Industrial Research (CSIR)
C.
Research Committee on Genetic Manipulation (RCGM)
D.
Genetic Engineering Appraisal Committee (GEAC)
Q169 Allen 3. PYQ MCQ
A 'new variety of rice was patented by a foreign company though such varieties have been present in India for a long time. This is related to :-
A.
Co-667
B.
Sharbati Sonora
C.
Lerma Rojo
D.
Basmati
Q170 Allen 3. PYQ MCQ
Use of bioresources by multinational companies and organisations without authorisation from the concerned country and its people is called :-
A.
Bio-infringement
B.
Biopiracy
C.
Biodegradation
D.
Bioexploitation
Q171 Allen 3. PYQ MCQ
The correct order of steps in Polymerase Chain Reaction ("PCR") is :-
A.
Extension, Denaturation, Annealing
B.
Annealing, Extension, Denaturation
C.
Denaturation, Extension, Annealing
D.
Denaturation, Annealing, Extension
Q172 Allen 3. PYQ MCQ
Which of the following is true for Golden rice?
A.
It is Vitamin A enriched, with a gene from daffodil
B.
It is pest resistant, with a gene from Bacillus thuringiensis
C.
It is drought tolerant, developed using Agrobacterium vector
D.
It has yellow grains, because of a gene introduced from a primitive variety of rice
Q173 Allen 3. PYQ MCQ
Which of the following features of genetic code does allow bacteria to produce human insulin by recombinant DNA technology?
A.
Genetic code is not ambiguous
B.
Genetic code is redundant
C.
Genetic code is nearly universal
D.
Genetic code is specific
Q174 Allen 3. PYQ MCQ
Following statements describe the characteristics of the enzyme Restriction endonuclease. Identify the incorrect statement:
A.
The enzyme cuts DNA molecule at identified position within the DNA
B.
The enzyme binds DNA at specific sites and cuts only one of the two strands.
C.
The enzyme cuts the sugar-phosphate backbone at specific sites on each strand.
D.
The enzyme recognizes a specific palindromic nucleotide sequence in the DNA
Q175 Allen 3. PYQ MCQ
DNA precipitation out of a mixture of biomolecules can be achieved by treatment with :-
A.
Isopropanol
B.
Chilled ethanol
C.
Methanol at room temperature
D.
Chilled chloroform