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Biotechnology And Its Applications

339 Questions Start Allen Test
All Exams Allen
Q126 Allen 3. PYQ MCQ
Agarose extracted from sea weeds finds use in:
A.
Spectrophotometry
B.
Tissue Culture
C.
"PCR"
D.
Gel electrophoresis
Q127 Allen 3. PYQ MCQ
Maximum number of existing transgenic animals is of :-
A.
Fish
B.
Mice
C.
Cow
D.
Pig
Q128 Allen 3. PYQ MCQ
The process of RNA interference has been used in the development of plants resistant to:
A.
Nematodes
B.
Fungi
C.
Viruses
D.
Insects
Q129 Allen 3. PYQ MCQ
Read the following four statements (A-D) about certain mistakes in two of them:-
(A) The first transgenic buffalo, Rosie produced milk which was human alphalactalbumin enriched. (B) Restriction enzymes are used in isolation of DNA from other macro molecules. (C) Downstream processing is one of the steps of R-DNA technology. (D) Disarmed pathogen vectors are also used in transfer of R-DNA into the host. Which are the two statements having mistakes ?
A.
Statements A and B
B.
Statements B and C
C.
Statements C and D
D.
StatementsA and C
Q130 Allen 3. PYQ MCQ
Silencing of mRNA has been used in producing transgenic plants resistant to:-
A.
Bacterial blights
B.
Bollworms
C.
Nematodes
D.
White rusts
Q131 Allen 3. PYQ MCQ
Bacillus thuringiensis forms protein crystals which contain insecticidal protein. This protein :-
A.
does not kill the carrier bacterium which is itself resistant to this toxin
B.
binds with epithelial cells of midgut of the insect pest ultimately killing it
C.
is coded by several genes including the gene cry
D.
is activated by acid pH of the foregut of
ect pest
Q132 Allen 3. PYQ MCQ
Which one of the following techniques made it possible to genetically engineer living organisms?
A.
Hybridization
B.
Recombinant DNA techniques
C.
X-ray diffraction
D.
Heavier isotope labelling
Q133 Allen 3. PYQ MCQ
Which one is a true statement regarding DNA polymerase used in "PCR" ?
A.
It is isolated from a virus
B.
It remains active at high temperature
C.
It is used to ligate introduced DNA in recipient cells
D.
It serves as a selectable marker
Q134 Allen 3. PYQ MCQ
For transformation, micro-particles coated with DNA to be bombarded with gene gun are made up of :-
A.
Silicon or Platinum
B.
Gold or Tungsten
C.
Silver or platinum
D.
Platinum or zinc
Q135 Allen 3. PYQ MCQ
The figure below is the diagrammatic representation of the E. coli vector pBR322. Which one of the given options correctly identifies its certain component(s)?
A.
Hind III, Eco RI-selectable markers
B.
ampR, tetR-antibiotic resistance genes
C.
ori-original restriction enzyme
D.
rop-reduced osmotic pressure
Q136 Allen 3. PYQ MCQ
Consumption of which one of the following foods can prevent the kind of blindness associated with vitamin 'A' deficiency?
A.
Golden rice
B.
Bt-Brinjal
C.
Flaver Savr 'tomato
D.
Canolla
Q137 Allen 3. PYQ MCQ
DNA or RNA segment tagged with a radiactive molecule is called :-
A.
Clone
B.
Plasmid
C.
Vector
D.
Probe
Q138 Allen 3. PYQ MCQ
Which one of the following represents a palindromic sequence in DNA ?
A.
$5 ^ { \prime } - \mathrm { C A T T A G } - 3 ^ { \prime }$ $5 ^ { \prime } - \mathrm { G A T A C C } - 3 ^ { \prime }$
B.
$3 ^ { \prime } - \mathsf { G A T A A C } - 5 ^ { \prime }$ $3 ^ { \prime } { \cdot } \operatorname { C C T A A G } - 5 ^ { \prime }$
C.
$5 ^ { \prime } - { \mathrm { G A A T T C } } - 3 ^ { \prime }$ $5 ^ { \prime } - \mathsf { C C A A T G } - 3 ^ { \prime }$
D.
$3 ^ { \prime } { \cdot } \mathsf { C T T A A G } { \cdot } 5 ^ { \prime }$ $3 ^ { \prime } - \mathrm { G A A T C C } - 5 ^ { \prime }$
Q139 Allen 3. PYQ MCQ
In genetic engineering, the antibiotics are used :-
A.
as sequences from where replication starts
B.
to keep the cultures free of infection
C.
as selectable markers
D.
to select healthy vectors
Q140 Allen 3. PYQ MCQ
The figure below shows three steps (A,B, C) of Polymerase Chain Reaction ("PCR"). Select the option giving correct identification together with what it represents?
A.
C-Extension in the presence of heat stable DNA polymerase
B.
A-Annealing with two sets of primers
C.
B-Denaturation at a temperature of about 98Ā°C separating the two DNA strands
D.
A-Denaturation at a temperature of about $5 0 ^ { \circ } \mathrm { C }$
Q141 Allen 3. PYQ MCQ
BioColumnics (gene-gun) is suitable for :-
A.
Constructing recombinant DNA by joining with vectors
B.
DNA finger printing
C.
Disarming pathogen vectors
D.
Transformation of plants cells
Q142 Allen 3. PYQ MCQ
Tobacco plants resistant to a nematode have been developed by the introduction of DNA that produced (in the host cells) :-
A.
an antifeedant
B.
a toxic protein
C.
both sense and anti-sense RNA
D.
a particular hormone
Q143 Allen 3. PYQ MCQ
The first clinical gene therapy was given for treating :-
A.
Rheumatoid arthritis
B.
Adenosine deaminase deficiency
C.
Diabetes mellitus
D.
Chicken pox
Q144 Allen 3. PYQ MCQ
The colonies of recombinant bacteria appear white in contrast to blue colonies of nonrecombinant bacteria because of :-
A.
Inactivation of glycosidase enzyme in recombinant bacteria
B.
Non-recombinant bacteria containing beta-galactosidase
C.
Insertional inactivation of alphagalactosidase in non-recombinant bacteria
D.
Insertional inactivation of betagalactosidase in recombinant bacteria
Q145 Allen 3. PYQ MCQ
DNA fragments generated by the restriction endonucleases in a chemical reaction can be separated by :-
A.
Restriction mapping
B.
Centrifugation
C.
Polymerase chain reaction
D.
Electrophoresis
Q146 Allen 3. PYQ MCQ
An analysis of chromosomal DNA using the Southern hybridization technique does not use:
A.
Electrophoresis
B.
Blotting
C.
Autoradiography
D.
"PCR"
Q147 Allen 3. PYQ MCQ
Which vector can clone only a small fragment of DNA?
A.
Bacterial artificial chromosome
B.
Yeast artificial chromosome
C.
Plasmid
D.
Cosmid
Q148 Allen 3. PYQ MCQ
The first human hormone produced by recombinant DNA technology is :-
A.
Insulin
B.
Estrogen
C.
Thyroxin
D.
Progesterone
Q149 Allen 3. PYQ MCQ
The crops engineered for glyphosate are resistant/tolerant to :-
A.
Bacteria
B.
Insects
C.
Herbicides
D.
Fungi
Q150 Allen 3. PYQ MCQ
In Bt cotton, the Bt toxin present in plant tissue as pro-toxin is converted into active toxin due to:-
A.
Acidic pH of the insect gut
B.
Action of gut micro-organisms
C.
Presence of conversion factors in insect gut
D.
Alkaline pH of the insect gut