Q51
Allen
1. PRINCIPLES AND PROCESSES
MCQ
'Transgenic' plants are produced by :-
A.
Inducing gene mutation
B.
Arresting spindle fibre formation
C.
Deleting sex chromosomes
D.
Introducing foreign genes
Q52
Allen
1. PRINCIPLES AND PROCESSES
MCQ
For a DNA to function as a cloning vector the most essential requirement is :-
A.
multiple restriction sites
B.
several selectable markers
C.
circular nature
D.
'ori' sequence
Q53
Allen
1. PRINCIPLES AND PROCESSES
MCQ
According to EFB, "The integration of natural science and organisms, cells, parts thereof and molecular analogues for products and services," is known as:-
A.
Biochemistry
B.
Bioinformatics
C.
Biotechnology
D.
Biology
Q54
Allen
1. PRINCIPLES AND PROCESSES
MCQ
The stickiness of DNA ends facilitates the action of which enzyme :-
A.
DNA polymerase
B.
DNA Ligase
C.
Restriction endonuclease
D.
Alkaline phosphatase
Q55
Allen
1. PRINCIPLES AND PROCESSES
MCQ
Which technique is used to check the progression of restriction enzyme digestion?
A.
""
B.
Gel electrophoresis
C.
Southern Blotting
D.
Staining
Q56
Allen
1. PRINCIPLES AND PROCESSES
MCQ
In gel electrophoresis, at which end of the gel the sample is loaded?
A.
In the wells
B.
Towards positive electrode
C.
Towards negative electrode
D.
1 & 3 both
Q57
Allen
1. PRINCIPLES AND PROCESSES
MCQ
An antibiotic resistance gene of plasmid vector which get inactivated due to insertion of alien DNA, helps in the selection of:-
A.
Transformants
B.
Recombinants
C.
Non-Transformants
Q58
Allen
1. PRINCIPLES AND PROCESSES
MCQ
In which type of bioreactor air bubbles dramatically increases the oxygen transfer area?
A.
Simple stirred tank bioreactor
B.
Sparged stirred tank bioreactor
C.
Both (1) & (2)
D.
None of these
Q59
Allen
1. PRINCIPLES AND PROCESSES
MCQ
Genetic modification (GM) has been used to:-
A.
Create tailor made plants
B.
Supply alternative resources to industries
C.
Enhanced nutritional value of food.
D.
All of the above
Q60
Allen
1. PRINCIPLES AND PROCESSES
MCQ
The choice of Bt-gene for experiment depends upon:-
A.
The host plant/crop
B.
Targeted pest/insect
C.
Bacillus strain
D.
1 & 2 both
Q61
Allen
1. PRINCIPLES AND PROCESSES
MCQ
In nematode resistance by RNA interference, some specific genes were introduced which form dsRNA. These were introduced in:-
A.
Nematode
B.
Host plant
C.
Agrobacterium
D.
All of these
Q62
Allen
1. PRINCIPLES AND PROCESSES
MCQ
Select the incorrect match:-
A.
Transgenic mice - Polio vaccine
B.
Rosie cow - $\alpha$-lactalbumin gene
C.
ssDNA/RNA probe - Gene therapy
D.
"PCR" - Molecular diagnosis
Q63
Allen
1. PRINCIPLES AND PROCESSES
MCQ
In EcoR I, R is stand for :-
A.
Strain
B.
Species
C.
Genus
D.
Order
Q64
Allen
1. PRINCIPLES AND PROCESSES
MCQ
Restriction endonucleases are used in genetic engineering to form:-
A.
Recombinant molecule of protein
B.
Recombinant molecule of DNA
C.
Recombinant molecule of protein & DNA
D.
Recombinant cell
Q65
Allen
1. PRINCIPLES AND PROCESSES
MCQ
Which instrument is used for the separation of DNA fragments?
A.
""
B.
Gel electrophoresis
C.
Bioreactor
D.
Restriction endonuclease
Q66
Allen
1. PRINCIPLES AND PROCESSES
MCQ
Which of following feature is not necessary for cloning vector?
A.
Origin of replication
B.
High copy number
C.
Selectable marker
D.
Cloning sites
Q67
Allen
1. PRINCIPLES AND PROCESSES
MCQ
Which of the following is not true for cloning vector?
A.
more than two origin site of replication
B.
vector should have selectable marker gene
C.
single recognition site for the commonly used restriction enzyme
D.
pBR-322 have tetracycline resistance gene
Q68
Allen
1. PRINCIPLES AND PROCESSES
MCQ
Transformation is a procedure through which:-
A.
A piece of DNA is introduced in a host bacterium
B.
A piece of DNA is introduced in a vector
C.
A piece of DNA is introduced from protein
D.
All of these
Q69
Allen
1. PRINCIPLES AND PROCESSES
MCQ
Second letter of the name of restriction endonuclease came from the:-
A.
Genus of organism
B.
Species of organism
C.
Family of organism
D.
Class of organism
Q70
Allen
1. PRINCIPLES AND PROCESSES
MCQ
To isolate DNA from fungi we have to break the wall. This is done by:-
A.
Lysozyme
B.
Cellulose
C.
Invertase
D.
Chitinase
Q71
Allen
1. PRINCIPLES AND PROCESSES
MCQ
Which of the following enzyme will get inactivated in insertional inactivation?
A.
Transacetylase
B.
Permease
C.
$\beta$-galactosidase
D.
Taq-polymerase
Q72
Allen
1. PRINCIPLES AND PROCESSES
MCQ
In presence of chromogenic substrate recombinant bacteria gives:-
A.
Red coloured colonies
B.
Colourless colonies
C.
Blue colonies
D.
Insulin
Q73
Allen
1. PRINCIPLES AND PROCESSES
MCQ
Which of the following enzyme is known as molecular scissors?
A.
Ligase
B.
DNA polymerase
C.
Restriction enzyme
D.
Helicase
Q74
Allen
1. PRINCIPLES AND PROCESSES
MCQ
Which of the following is not required in "PCR"?
A.
DNA primer
B.
DNA template
C.
RNA primer
D.
Taq polymerase
Q75
Allen
1. PRINCIPLES AND PROCESSES
MCQ
The substrate for restriction enzyme is:-
A.
Single stranded RNA
B.
Proteins
C.
Double stranded DNA
D.
Single stranded DNA