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Biotechnology And Its Applications

339 Questions Start Allen Test
All Exams Allen
Q251 Allen 4. NCERT BASED QUESTIONS MCQ
The linking of antibiotic resistant gene in the plasmid vector become possible with the enzyme :-
A.
Restriction endonuclease
B.
DNA ligase
C.
DNA polymerase
D.
RNA polymerase
Q252 Allen 4. NCERT BASED QUESTIONS MCQ
If any protein encoding gene is expressed in a heterologous host then protein is known as:-
A.
Recombinant gene
B.
Recombinant protein
C.
Selectable marker
D.
Homogenous protein
Q253 Allen 4. NCERT BASED QUESTIONS MCQ
The normal E. coli cell carries resistance gene against:-
A.
Ampicillin
B.
Chloramphenicol
C.
Tetracycline
D.
None of the above
Q254 Allen 4. NCERT BASED QUESTIONS MCQ
Taq polymerase is used in, polymerase chain reaction, because:-
A.
It becomes inactive at high temperature
B.
It makes other enzyme active at high temperature
C.
It remains active during high temperature
D.
It is obtained from thermostable virus
Q255 Allen 4. NCERT BASED QUESTIONS MCQ
The vessels, where large volumes of culture can be processed are:-
A.
Bioreactors
B.
Biovessels
C.
Biocontainers
D.
All of above
Q256 Allen 4. NCERT BASED QUESTIONS MCQ
Small chemically synthesised oligonucleotides that are complementary to the regions of DNA at 3' end used in "PCR" are:-
A.
Primers
B.
Dimers
C.
Small strands
D.
Large fragments
Q257 Allen 4. NCERT BASED QUESTIONS MCQ
Bombardment of high velocity micro-particles of gold or tungsten coated with DNA on target cells is:-
A.
BioColumnics
B.
Micro-injection
C.
Electroporation
D.
Bombing
Q258 Allen 4. NCERT BASED QUESTIONS MCQ
In micro injection:-
A.
DNA is bombarded on target cells
B.
DNA is placed through a vector
C.
DNA is directly injected into the nucleus of animal cell
D.
None of the above
Q259 Allen 4. NCERT BASED QUESTIONS MCQ
Most common matrix is agarose a natural polymer used in gel electrophoresis is extracted from:-
A.
an animal
B.
a fungus
C.
sea weeds
D.
None of these
Q260 Allen 4. NCERT BASED QUESTIONS MCQ
Agrobacterium tumifaciens a pathogen transform normal plant cells into a tumor, similarly in animals the normal cells transformed into cancerous cells by:-
A.
Retro viruses
B.
DNA viruses
C.
Ribo viruses
D.
None of these
Q261 Allen 4. NCERT BASED QUESTIONS MCQ
Insertional inactivation results into inactivation of which enzyme?
A.
Transacetylase
B.
Permease
C.
Taq polymerase
D.
$\beta$-galactosidase
Q262 Allen 4. NCERT BASED QUESTIONS MCQ
If the bacterium does not have any insert, then the presence of chromogenic substrate, it gives:-
A.
Red coloured colonies
B.
Colourless colonies
C.
Blue colonies
D.
Green colonies
Q263 Allen 4. NCERT BASED QUESTIONS MCQ
To make cell competent to take up DNA, heat shock is given to cells, the temperature of shock is:-
A.
30Ā°C
B.
42Ā°C
C.
60Ā°C
D.
90Ā°C
Q264 Allen 4. NCERT BASED QUESTIONS MCQ
In gel electrophoresis technique the DNA fragments are forced to move through a medium towards:-
A.
Anode
B.
Cathode
C.
Both (1) and (2)
D.
None of the above
Q265 Allen 4. NCERT BASED QUESTIONS MCQ
Which one is not a basic step in genetically modifying an organism?
A.
Identification of DNA with desirable genes
B.
Introduction of the identified DNA into the host
C.
Introduction of unidentified DNA into the host
D.
Maintenance of introduced DNA in the host and transfer of the DNA to its progeny
Q266 Allen 4. NCERT BASED QUESTIONS MCQ
The most commonly used bioreactors are of:-
A.
Simple stirring type
B.
Sparged stirring type
C.
Both 1 and 2
D.
None of the above
Q267 Allen 4. NCERT BASED QUESTIONS MCQ
Downstream processing is:-
A.
Process of separation of DNA fragments
B.
Process of joining the vector and the host DNA
C.
Process including separation and purification of the product
D.
Process of transferring DNA.
Q268 Allen 4. NCERT BASED QUESTIONS MCQ
Taq. polymerase is obtained from :-
A.
Bacillus thuriengiensis
B.
Thermus aquaticus
C.
Salmonella typhimurium
D.
Eischerichia coli
Q269 Allen 4. NCERT BASED QUESTIONS MCQ
To denature the DNA template in "PCR" it is heated to
A.
70Ā°C
B.
54Ā°C
C.
80Ā°C
D.
94Ā°C
Q270 Allen 4. NCERT BASED QUESTIONS MCQ
Alternative selectable markers developed to differentiate non-recombinants from recombinants on the basis of :-
A.
Ability of separate them according to size
B.
Ability to produce colour in the presence of a chromogenic substrate
C.
Ability to not produce colour
D.
None of the above
Q271 Allen 4. NCERT BASED QUESTIONS MCQ
If DNA is inserted within the coding sequence of $\beta$ -galactosidase enzyme then
A.
Non-recombinants will give blue coloured colonies in presence of chromogenic substrate
B.
Recombinant will give blue coloured colonies in presence of chromogenic substrate
C.
Both recombinants and nonrecombinants give blue colour
D.
Non-recombinants do not produce colour due to insertional inactivation.
Q272 Allen 4. NCERT BASED QUESTIONS MCQ
In gel electrophoresis the DNA fragments separate according to size (smaller the fragment size, the faster it moves) this effect is called :-
A.
Sieving effect
B.
Movement effect
C.
Size effect
D.
Spooling
Q273 Allen 4. NCERT BASED QUESTIONS MCQ
Extraction, purification and packaging of products is collectively known as :-
A.
Upstream processing
B.
Distillation
C.
Downstream processing
D.
Genetic engineering
Q274 Allen 4. NCERT BASED QUESTIONS MCQ
You have three copies of a particular DNA molecule what technique would you use to make more copies of the molecule?
A.
Gel electrophoresis
B.
Sequencing
C.
"PCR"
D.
Restriction fragment analysis
Q275 Allen 4. NCERT BASED QUESTIONS MCQ
Ti plasmid is present in :-
A.
E.coli
B.
Agrobacterium tumefaciens
C.
Agrobacterium orifaciens
D.
Vibrio cholera