Biotechnology And Its Applications

339 Questions Start Allen Test
Q226 Allen 4. NCERT BASED QUESTIONS MCQ
Autonomously replicating circular extra chromosomal DNA of bacteria is :-
A.
Plastid
B.
Nucleus
C.
Plasmid
D.
None of these
Q227 Allen 4. NCERT BASED QUESTIONS MCQ
The specific DNA sequence in a chromosome which is responsible for initiation of replication is :-
A.
Cloning region
B.
Termination region
C.
Initiation region
D.
Origin of replication
Q228 Allen 4. NCERT BASED QUESTIONS MCQ
Which of the following enzymes is known as 'genetic glue'?
A.
DNA polymerase
B.
Alkaline phosphatase
C.
DNA ligase
D.
All of the above
Q229 Allen 4. NCERT BASED QUESTIONS MCQ
pBR322 has two antibiotic resistance genes, they are :-
A.
Streptomycin and Ampicillin resistant gene
B.
Chloromycetin and tetracycline resistant gene
C.
Tetracycline and neomycin resistant genes
D.
Ampicillin and tetracyclin resistant genes
Q230 Allen 4. NCERT BASED QUESTIONS MCQ
To isolate DNA from the plant cells we have to break the wall this is done by :-
A.
Lysozyme
B.
Cellulase
C.
Chitinase
D.
Invertase
Q231 Allen 4. NCERT BASED QUESTIONS MCQ
Restriction enzymes belong to a larger class of enzymes called :-
A.
Cellulases
B.
Hydrolases
C.
Polymerases
D.
Nucleases
Q232 Allen 4. NCERT BASED QUESTIONS MCQ
The construction of the first recombinant DNA was done by ?
A.
Stanley cohen and Herbert Boyer
B.
Nathan's and Smith
C.
Maeselson and Stahl
D.
Allec Jeffreys
Q233 Allen 4. NCERT BASED QUESTIONS MCQ
EcoR I recognises palindromic sequence:-
A.
5'GGGCCC3' 3'CCCGGG
B.
5'-GAATTC-3' 3-CTTAAG-5'
C.
5-AAGCTT3' 3-TTCGAA-5
D.
None of the above
Q234 Allen 4. NCERT BASED QUESTIONS MCQ
Vector which is commonly used to transfer foreign gene in a crop plant is :-
A.
Plasmids of Salmonella
B.
$\lambda\text{ bacteriophage vector}$
C.
Ti plasmid of Agrobacterium tumifaciens
D.
None of the above
Q235 Allen 4. NCERT BASED QUESTIONS MCQ
Father of genetic engineering is :-
A.
Paul Berg
B.
Nathans
C.
Herbert Boyer
D.
Stanley Cohen
Q236 Allen 4. NCERT BASED QUESTIONS MCQ
A definition of biotechnology that encompasses both traditional view and modern view are given by :-
A.
European forum on Biotechnology
B.
European focus on Biotechnology
C.
European Federation of Biotechnology
D.
European Centre of Biotechnology
Q237 Allen 4. NCERT BASED QUESTIONS MCQ
Which one of the following is must in Genetic engineering?
A.
Restriction endonuclease + DNA ligase
B.
Restriction exonuclease + DNA polymerase
C.
Alkaline phosphate + DNA Ligase
D.
RNA polymerase + DNA polymerase
Q238 Allen 4. NCERT BASED QUESTIONS MCQ
Roman numbers following the names of restriction endonuclease indicate :-
A.
The order in which the enzymes were isolated from that strain of bacteria
B.
strain of bacteria
C.
the order in which genus is taken to isolate the enzyme
D.
none of the above
Q239 Allen 4. NCERT BASED QUESTIONS MCQ
Exonuclease removes nucleotides from:-
A.
Specific positions
B.
the ends of the DNA
C.
any where in DNA
D.
All the above
Q240 Allen 4. NCERT BASED QUESTIONS MCQ
In a chromosome there is a specific DNA sequence which is responsible for initiating replication is :-
A.
Ori
B.
Palindromic sequence
C.
Initiation sequence
D.
Promoter sequence
Q241 Allen 4. NCERT BASED QUESTIONS MCQ
The first restriction endonuclease discovered was:-
A.
EcoR I
B.
Sam I
C.
BamH I
D.
Hind II
Q242 Allen 4. NCERT BASED QUESTIONS MCQ
In the vector pBR322 there is :-
A.
One selectable marker
B.
Two selectable markers
C.
Three selectable markers
D.
None of the above
Q243 Allen 4. NCERT BASED QUESTIONS MCQ
When the isolation of genetic material is done the RNA can be removed by treatment with :-
A.
Protease
B.
Chitinase
C.
Ribonuclease
D.
Deoxyribonuclease
Q244 Allen 4. NCERT BASED QUESTIONS MCQ
Knife of DNA :-
A.
DNA - ligase
B.
Restriction endonuclease
C.
Exonuclease
D.
Peptidase
Q245 Allen 4. NCERT BASED QUESTIONS MCQ
The enzymes, which remove nucleotides from the ends of the DNA are :-
A.
Exonuclease
B.
Endonuclease
C.
Cellulase
D.
Hydrolase
Q246 Allen 4. NCERT BASED QUESTIONS MCQ
Group of letters that form the same words when read both forward and backward is called :-
A.
Palindrome
B.
Same words
C.
Opposite words
D.
None of the above
Q247 Allen 4. NCERT BASED QUESTIONS MCQ
Which type of ends are produced by EcoR I?
A.
Blunt ends
B.
Sticky ends
C.
Both (1) and (2)
D.
None of the above
Q248 Allen 4. NCERT BASED QUESTIONS MCQ
The sequence which is responsible for controlling the copy number of the linked DNA is :-
A.
Coding sequence
B.
Promoter sequence
C.
Terminator sequence
D.
Ori
Q249 Allen 4. NCERT BASED QUESTIONS MCQ
Apart from DNA in the bacterial nucleoid, there is a circular extrachromosomal DNA in a bacterial cell called :-
A.
Plasmid
B.
Mesosomes
C.
Chromosome
D.
None of these
Q250 Allen 4. NCERT BASED QUESTIONS MCQ
The stickiness of the ends, facilitates the action of enzyme :-
A.
DNA ligase
B.
DNA polymerase
C.
Alkaline phosphatase
D.
All of the above