Q226
Allen
4. NCERT BASED QUESTIONS
MCQ
Autonomously replicating circular extra chromosomal DNA of bacteria is :-
A.
Plastid
B.
Nucleus
C.
Plasmid
D.
None of these
Q227
Allen
4. NCERT BASED QUESTIONS
MCQ
The specific DNA sequence in a chromosome which is responsible for initiation of replication is :-
A.
Cloning region
B.
Termination region
C.
Initiation region
D.
Origin of replication
Q228
Allen
4. NCERT BASED QUESTIONS
MCQ
Which of the following enzymes is known as 'genetic glue'?
A.
DNA polymerase
B.
Alkaline phosphatase
C.
DNA ligase
D.
All of the above
Q229
Allen
4. NCERT BASED QUESTIONS
MCQ
pBR322 has two antibiotic resistance genes, they are :-
A.
Streptomycin and Ampicillin resistant gene
B.
Chloromycetin and tetracycline resistant gene
C.
Tetracycline and neomycin resistant genes
D.
Ampicillin and tetracyclin resistant genes
Q230
Allen
4. NCERT BASED QUESTIONS
MCQ
To isolate DNA from the plant cells we have to break the wall this is done by :-
A.
Lysozyme
B.
Cellulase
C.
Chitinase
D.
Invertase
Q231
Allen
4. NCERT BASED QUESTIONS
MCQ
Restriction enzymes belong to a larger class of enzymes called :-
A.
Cellulases
B.
Hydrolases
C.
Polymerases
D.
Nucleases
Q232
Allen
4. NCERT BASED QUESTIONS
MCQ
The construction of the first recombinant DNA was done by ?
A.
Stanley cohen and Herbert Boyer
B.
Nathan's and Smith
C.
Maeselson and Stahl
D.
Allec Jeffreys
Q233
Allen
4. NCERT BASED QUESTIONS
MCQ
EcoR I recognises palindromic sequence:-
A.
5'GGGCCC3' 3'CCCGGG
B.
5'-GAATTC-3' 3-CTTAAG-5'
C.
5-AAGCTT3' 3-TTCGAA-5
D.
None of the above
Q234
Allen
4. NCERT BASED QUESTIONS
MCQ
Vector which is commonly used to transfer foreign gene in a crop plant is :-
A.
Plasmids of Salmonella
B.
$\lambda\text{ bacteriophage vector}$
C.
Ti plasmid of Agrobacterium tumifaciens
D.
None of the above
Q235
Allen
4. NCERT BASED QUESTIONS
MCQ
Father of genetic engineering is :-
A.
Paul Berg
B.
Nathans
C.
Herbert Boyer
D.
Stanley Cohen
Q236
Allen
4. NCERT BASED QUESTIONS
MCQ
A definition of biotechnology that encompasses both traditional view and modern view are given by :-
A.
European forum on Biotechnology
B.
European focus on Biotechnology
C.
European Federation of Biotechnology
D.
European Centre of Biotechnology
Q237
Allen
4. NCERT BASED QUESTIONS
MCQ
Which one of the following is must in Genetic engineering?
A.
Restriction endonuclease + DNA ligase
B.
Restriction exonuclease + DNA polymerase
C.
Alkaline phosphate + DNA Ligase
D.
RNA polymerase + DNA polymerase
Q238
Allen
4. NCERT BASED QUESTIONS
MCQ
Roman numbers following the names of restriction endonuclease indicate :-
A.
The order in which the enzymes were isolated from that strain of bacteria
B.
strain of bacteria
C.
the order in which genus is taken to isolate the enzyme
D.
none of the above
Q239
Allen
4. NCERT BASED QUESTIONS
MCQ
Exonuclease removes nucleotides from:-
A.
Specific positions
B.
the ends of the DNA
C.
any where in DNA
D.
All the above
Q240
Allen
4. NCERT BASED QUESTIONS
MCQ
In a chromosome there is a specific DNA sequence which is responsible for initiating replication is :-
A.
Ori
B.
Palindromic sequence
C.
Initiation sequence
D.
Promoter sequence
Q241
Allen
4. NCERT BASED QUESTIONS
MCQ
The first restriction endonuclease discovered was:-
A.
EcoR I
B.
Sam I
C.
BamH I
D.
Hind II
Q242
Allen
4. NCERT BASED QUESTIONS
MCQ
In the vector pBR322 there is :-
A.
One selectable marker
B.
Two selectable markers
C.
Three selectable markers
D.
None of the above
Q243
Allen
4. NCERT BASED QUESTIONS
MCQ
When the isolation of genetic material is done the RNA can be removed by treatment with :-
A.
Protease
B.
Chitinase
C.
Ribonuclease
D.
Deoxyribonuclease
Q244
Allen
4. NCERT BASED QUESTIONS
MCQ
Knife of DNA :-
A.
DNA - ligase
B.
Restriction endonuclease
C.
Exonuclease
D.
Peptidase
Q245
Allen
4. NCERT BASED QUESTIONS
MCQ
The enzymes, which remove nucleotides from the ends of the DNA are :-
A.
Exonuclease
B.
Endonuclease
C.
Cellulase
D.
Hydrolase
Q246
Allen
4. NCERT BASED QUESTIONS
MCQ
Group of letters that form the same words when read both forward and backward is called :-
A.
Palindrome
B.
Same words
C.
Opposite words
D.
None of the above
Q247
Allen
4. NCERT BASED QUESTIONS
MCQ
Which type of ends are produced by EcoR I?
A.
Blunt ends
B.
Sticky ends
C.
Both (1) and (2)
D.
None of the above
Q248
Allen
4. NCERT BASED QUESTIONS
MCQ
The sequence which is responsible for controlling the copy number of the linked DNA is :-
A.
Coding sequence
B.
Promoter sequence
C.
Terminator sequence
D.
Ori
Q249
Allen
4. NCERT BASED QUESTIONS
MCQ
Apart from DNA in the bacterial nucleoid, there is a circular extrachromosomal DNA in a bacterial cell called :-
A.
Plasmid
B.
Mesosomes
C.
Chromosome
D.
None of these
Q250
Allen
4. NCERT BASED QUESTIONS
MCQ
The stickiness of the ends, facilitates the action of enzyme :-
A.
DNA ligase
B.
DNA polymerase
C.
Alkaline phosphatase
D.
All of the above